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dc.contributor.authorPerozziello, Gerardo
dc.contributor.authorCandeloro, Patrizio
dc.contributor.authorGentile, Francesco T.
dc.contributor.authorColuccio, Maria Laura
dc.contributor.authorTallerico, Marco
dc.contributor.authorDe Grazia, Antonio
dc.contributor.authorNicastri, Annalisa
dc.contributor.authorPerri, Angela Mena
dc.contributor.authorParrotta, Elvira
dc.contributor.authorPardeo, Francesca
dc.contributor.authorCatalano, Rossella
dc.contributor.authorCuda, Giovanni
dc.contributor.authorDi Fabrizio, Enzo M.
dc.date.accessioned2015-08-03T12:35:57Z
dc.date.available2015-08-03T12:35:57Z
dc.date.issued2015-08
dc.identifier.issn01679317
dc.identifier.doi10.1016/j.mee.2015.02.015
dc.identifier.urihttp://hdl.handle.net/10754/564194
dc.description.abstractIn this paper, we present a microfluidic device fabricated with a simple and inexpensive process allowing rapid filtering of peptides from a complex mixture. The polymer microfluidic device can be used for sample preparation in biological applications. The device is fabricated by micromilling and solvent assisted bonding, in which a microdialysis membrane (cut-off of 12-14 kDa) is sandwiched in between an upper and a bottom microfluidic chamber. An external frame connects the microfluidic device to external tubes, microvalves and syringe pumps. Bonding strength and interface sealing are pneumatically tested. Microfluidic protocols are also described by using the presented device to filter a sample composed of specific peptides (MW 1553.73 Da, at a concentration of 1.0 ng/μl) derived from the BRCA1 protein, a tumor-suppressor molecule which plays a pivotal role in the development of breast cancer, and albumin (MW 66.5 kDa, at a concentration of 35 μg/μl), the most represented protein in human plasma. The filtered samples coming out from the microfluidic device were subsequently deposited on a SERS (surface enhanced Raman scattering) substrate for further analysis by Raman spectroscopy. By using this approach, we were able to sort the small peptides from the bigger and highly concentrated protein albumin and to detect them by using a label-free technique at a resolution down to 1.0 ng/μl.
dc.publisherElsevier BV
dc.subjectComplex mixture analysis
dc.subjectMicrofluidic filtering
dc.subjectSERS analysis
dc.titleA microfluidic dialysis device for complex biological mixture SERS analysis
dc.typeArticle
dc.contributor.departmentMaterial Science and Engineering Program
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalMicroelectronic Engineering
dc.contributor.institutionBioNEM Lab, Department of Experimental and Clinical Medicine, University Magna Graecia of CatanzaroCatanzaro, Italy
dc.contributor.institutionProteomics Lab, Department of Experimental and Clinical Medicine, University Magna Graecia of CatanzaroCatanzaro, Italy
kaust.personTallerico, Marco
kaust.personDe Grazia, Antonio
kaust.personDi Fabrizio, Enzo M.


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