A microfluidic dialysis device for complex biological mixture SERS analysis
Type
ArticleAuthors
Perozziello, GerardoCandeloro, Patrizio

Gentile, Francesco T.
Coluccio, Maria Laura
Tallerico, Marco

De Grazia, Antonio

Nicastri, Annalisa
Perri, Angela Mena
Parrotta, Elvira
Pardeo, Francesca
Catalano, Rossella
Cuda, Giovanni

Di Fabrizio, Enzo M.

KAUST Department
Material Science and Engineering ProgramPhysical Science and Engineering (PSE) Division
Date
2015-08Permanent link to this record
http://hdl.handle.net/10754/564194
Metadata
Show full item recordAbstract
In this paper, we present a microfluidic device fabricated with a simple and inexpensive process allowing rapid filtering of peptides from a complex mixture. The polymer microfluidic device can be used for sample preparation in biological applications. The device is fabricated by micromilling and solvent assisted bonding, in which a microdialysis membrane (cut-off of 12-14 kDa) is sandwiched in between an upper and a bottom microfluidic chamber. An external frame connects the microfluidic device to external tubes, microvalves and syringe pumps. Bonding strength and interface sealing are pneumatically tested. Microfluidic protocols are also described by using the presented device to filter a sample composed of specific peptides (MW 1553.73 Da, at a concentration of 1.0 ng/μl) derived from the BRCA1 protein, a tumor-suppressor molecule which plays a pivotal role in the development of breast cancer, and albumin (MW 66.5 kDa, at a concentration of 35 μg/μl), the most represented protein in human plasma. The filtered samples coming out from the microfluidic device were subsequently deposited on a SERS (surface enhanced Raman scattering) substrate for further analysis by Raman spectroscopy. By using this approach, we were able to sort the small peptides from the bigger and highly concentrated protein albumin and to detect them by using a label-free technique at a resolution down to 1.0 ng/μl.Citation
Perozziello, G., Candeloro, P., Gentile, F., Coluccio, M. L., Tallerico, M., De Grazia, A., … Di Fabrizio, E. (2015). A microfluidic dialysis device for complex biological mixture SERS analysis. Microelectronic Engineering, 144, 37–41. doi:10.1016/j.mee.2015.02.015Publisher
Elsevier BVJournal
Microelectronic Engineeringae974a485f413a2113503eed53cd6c53
10.1016/j.mee.2015.02.015