Dynamics of bacterial communities before and after distribution in a full-scale drinking water network
AuthorsEl Chakhtoura, Joline
Prest, Emmanuelle I E C
van Loosdrecht, Mark C.M.
Hammes, Frederik A.
Vrouwenvelder, Johannes S.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Environmental Biotechnology Research Group
Environmental Science and Engineering Program
Water Desalination and Reuse Research Center (WDRC)
Permanent link to this recordhttp://hdl.handle.net/10754/564158
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AbstractUnderstanding the biological stability of drinking water distribution systems is imperative in the framework of process control and risk management. The objective of this research was to examine the dynamics of the bacterial community during drinking water distribution at high temporal resolution. Water samples (156 in total) were collected over short time-scales (minutes/hours/days) from the outlet of a treatment plant and a location in its corresponding distribution network. The drinking water is treated by biofiltration and disinfectant residuals are absent during distribution. The community was analyzed by 16S rRNA gene pyrosequencing and flow cytometry as well as conventional, culture-based methods. Despite a random dramatic event (detected with pyrosequencing and flow cytometry but not with plate counts), the bacterial community profile at the two locations did not vary significantly over time. A diverse core microbiome was shared between the two locations (58-65% of the taxa and 86-91% of the sequences) and found to be dependent on the treatment strategy. The bacterial community structure changed during distribution, with greater richness detected in the network and phyla such as Acidobacteria and Gemmatimonadetes becoming abundant. The rare taxa displayed the highest dynamicity, causing the major change during water distribution. This change did not have hygienic implications and is contingent on the sensitivity of the applied methods. The concept of biological stability therefore needs to be revised. Biostability is generally desired in drinking water guidelines but may be difficult to achieve in large-scale complex distribution systems that are inherently dynamic.
SponsorsThis research was supported by Evides Waterbedrijf and King Abdullah University of Science and Technology (KAUST) funding. The authors thank Lip-Smacking Design for their artwork services.
- Combining flow cytometry and 16S rRNA gene pyrosequencing: a promising approach for drinking water monitoring and characterization.
- Authors: Prest EI, El-Chakhtoura J, Hammes F, Saikaly PE, van Loosdrecht MC, Vrouwenvelder JS
- Issue date: 2014 Oct 15
- A microbiology-based multi-parametric approach towards assessing biological stability in drinking water distribution networks.
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- Issue date: 2013 Jun 1
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- Issue date: 2018 Aug 1
- Long-Term Bacterial Dynamics in a Full-Scale Drinking Water Distribution System.
- Authors: Prest EI, Weissbrodt DG, Hammes F, van Loosdrecht MC, Vrouwenvelder JS
- Issue date: 2016
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- Authors: Li C, Ling F, Zhang M, Liu WT, Li Y, Liu W
- Issue date: 2017 Jan