Allochthonous bioaugmentation in ex situ treatment of crude oil-polluted sediments in the presence of an effective degrading indigenous microbiome
Antoniou, E. A.
Hussein, Emad I.
Al-Horani, Fuad A.
Malkawi, Hanan Issa
Abdel-Fattah, Yasser Refaat
Kalogerakis, Nicolas E.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Extreme Systems Microbiology Lab
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AbstractOil-polluted sediment bioremediation depends on both physicochemical and biological parameters, but the effect of the latter cannot be evaluated without the optimization of the former. We aimed in optimizing the physicochemical parameters related to biodegradation by applying an ex-situ landfarming set-up combined with biostimulation to oil-polluted sediment, in order to determine the added effect of bioaugmentation by four allochthonous oil-degrading bacterial consortia in relation to the degradation efficiency of the indigenous community. We monitored hydrocarbon degradation, sediment ecotoxicity and hydrolytic activity, bacterial population sizes and bacterial community dynamics, characterizing the dominant taxa through time and at each treatment. We observed no significant differences in total degradation, but increased ecotoxicity between the different treatments receiving both biostimulation and bioaugmentation and the biostimulated-only control. Moreover, the added allochthonous bacteria quickly perished and were rarely detected, their addition inducing minimal shifts in community structure although it altered the distribution of the residual hydrocarbons in two treatments. Therefore, we concluded that biodegradation was mostly performed by the autochthonous populations while bioaugmentation, in contrast to biostimulation, did not enhance the remediation process. Our results indicate that when environmental conditions are optimized, the indigenous microbiome at a polluted site will likely outperform any allochthonous consortium.
SponsorsThis work was funded by FP-7 PROJECT No. 266473, "Unravelling and exploiting Mediterranean Sea microbial diversity and ecology for xenobiotics' and pollutants' clean up" - ULIXES. The authors would like to thank Prof. Nico Boon for his valuable advice and the five anonymous reviewers for their constructive comments. Francesca Mapelli was supported by Universita degli Studi di Milano, DeFENS, European Social Found (FSE) and Regione Lombardia (contract "Dote Ricerca").
JournalJournal of Hazardous Materials
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