Show simple item record

dc.contributor.authorRodighiero, Simona
dc.contributor.authorTorre, Bruno
dc.contributor.authorSogne, Elisa
dc.contributor.authorRuffilli, Roberta
dc.contributor.authorCagnoli, Cinzia
dc.contributor.authorFrancolini, Maura
dc.contributor.authorDi Fabrizio, Enzo M.
dc.contributor.authorFalqui, Andrea
dc.date.accessioned2015-08-03T12:32:31Z
dc.date.available2015-08-03T12:32:31Z
dc.date.issued2015-03-22
dc.identifier.issn1059910X
dc.identifier.pmid25810353
dc.identifier.doi10.1002/jemt.22492
dc.identifier.urihttp://hdl.handle.net/10754/564109
dc.description.abstractConfocal microscopy imaging of cells allows to visualize the presence of specific antigens by using fluorescent tags or fluorescent proteins, with resolution of few hundreds of nanometers, providing their localization in a large field-of-view and the understanding of their cellular function. Conversely, in scanning electron microscopy (SEM), the surface morphology of cells is imaged down to nanometer scale using secondary electrons. Combining both imaging techniques have brought to the correlative light and electron microscopy, contributing to investigate the existing relationships between biological surface structures and functions. Furthermore, in SEM, backscattered electrons (BSE) can image local compositional differences, like those due to nanosized gold particles labeling cellular surface antigens. To perform SEM imaging of cells, they could be grown on conducting substrates, but obtaining images of limited quality. Alternatively, they could be rendered electrically conductive, coating them with a thin metal layer. However, when BSE are collected to detect gold-labeled surface antigens, heavy metals cannot be used as coating material, as they would mask the BSE signal produced by the markers. Cell surface could be then coated with a thin layer of chromium, but this results in a loss of conductivity due to the fast chromium oxidation, if the samples come in contact with air. In order to overcome these major limitations, a thin layer of indium-tin-oxide was deposited by ion-sputtering on gold-decorated HeLa cells and neurons. Indium-tin-oxide was able to provide stable electrical conductivity and preservation of the BSE signal coming from the gold-conjugated markers. © 2015 Wiley Periodicals, Inc.
dc.publisherWiley
dc.subjectConfocal microscopy
dc.subjectCorrelative microscopy
dc.subjectImmunolabeling
dc.subjectITO
dc.subjectScanning electron microscopy
dc.titleCorrelative scanning electron and confocal microscopy imaging of labeled cells coated by indium-tin-oxide
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentMaterial Science and Engineering Program
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalMicroscopy Research and Technique
dc.contributor.institutionFondazione Filarete, Viale Ortles 22/4, Milano, Italy
dc.contributor.institutionCEMES/CNRS, 29 Rue Jeanne Marvig BP 94347, Toulouse Cedex 4, France
dc.contributor.institutionDepartment of Medical Biotechnology and Translational Medicine, Università Degli Studi Di Milano, Milano, Italy
kaust.personTorre, Bruno
kaust.personSogne, Elisa
kaust.personDi Fabrizio, Enzo M.
kaust.personFalqui, Andrea
dc.date.published-online2015-03-22
dc.date.published-print2015-06


This item appears in the following Collection(s)

Show simple item record