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dc.contributor.authorFlegontov, Pavel
dc.contributor.authorMichálek, Jan
dc.contributor.authorJanouškovec, Jan
dc.contributor.authorLai, De Hua
dc.contributor.authorJirků, Milan
dc.contributor.authorHajdušková, Eva
dc.contributor.authorTomčala, Aleš
dc.contributor.authorOtto, Thomas D.
dc.contributor.authorKeeling, Patrick J.
dc.contributor.authorPain, Arnab
dc.contributor.authorOborník, Miroslav
dc.contributor.authorLukeš, J.
dc.date.accessioned2015-08-03T12:29:47Z
dc.date.available2015-08-03T12:29:47Z
dc.date.issued2015-02-06
dc.identifier.issn07374038
dc.identifier.pmid25660376
dc.identifier.doi10.1093/molbev/msv021
dc.identifier.urihttp://hdl.handle.net/10754/564048
dc.description.abstractFour respiratory complexes and ATP-synthase represent central functional units in mitochondria. In some mitochondria and derived anaerobic organelles, a few or all of these respiratory complexes have been lost during evolution. We show that the respiratory chain of Chromera velia, a phototrophic relative of parasitic apicomplexans, lacks complexes I and III, making it a uniquely reduced aerobic mitochondrion. In Chromera, putative lactate:cytochrome c oxidoreductases are predicted to transfer electrons from lactate to cytochrome c, rendering complex III unnecessary. The mitochondrial genome of Chromera has the smallest known protein-coding capacity of all mitochondria, encoding just cox1 and cox3 on heterogeneous linear molecules. In contrast, another photosynthetic relative of apicomplexans, Vitrella brassicaformis, retains the same set of genes as apicomplexans and dinoflagellates (cox1, cox3, and cob). © The Author 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.
dc.description.sponsorshipThe authors thank the KAUST Bioscience Core Laboratory personnel for sequencing Illumina libraries used in this project, Evgeny S. Gerasimov (Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow) and Martin Kolisko (University of British Columbia, Vancouver) for help with sequence analysis, Oldrich Benada (Institute of Microbiology, Prague) for help with electron microscopy, Anton Horvath (Comenius University, Bratislava), Dave Speijer (University of Amsterdam, Amsterdam), and Ivan Hrdy (Charles University, Prague) for useful comments. This work was supported by Czech Science Foundation grants P506/12/1522, 13-33039S (support for J.M.) and P501/12/G055 (support for A.T.) to M.O., project Algatech (CZ.1.05/2.1.00/03.0110) to M.O., FP7 agreement 316304 to M.O. and J.L., the KAUST award FIC/2010/09 to A.P., M.O. and J.L., and by a grant from the Canadian Institutes for Health Research to P.J.K. and J.L. is also supported by the Praemium Academiae, and P.F. by a grant of the Moravian-Silesian region (project MSK2013-DT1) and by Russian Foundation for Basic Research (project 14-04-31936). P.J.K. and J.L. are Senior Fellows and J.J. is a Global Scholar at the Canadian Institute for Advanced Research, and P.J.K. was supported by a Fellowship from the John Simon Guggenheim Foundation.
dc.publisherOxford University Press (OUP)
dc.subjectanaerobic metabolism
dc.subjectApicomplexa
dc.subjectChromera
dc.subjectevolution
dc.subjectrespiratory chain
dc.subjectVitrella
dc.titleDivergent mitochondrial respiratory chains in phototrophic relatives of apicomplexan parasites
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentPathogen Genomics Laboratory
dc.identifier.journalMolecular Biology and Evolution
dc.contributor.institutionInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic
dc.contributor.institutionLife Science Research Centre, Department of Biology and Ecology, University of Ostrava, Ostrava, Czech Republic
dc.contributor.institutionFaculty of Science, University of South Bohemia, České Budějovice, Czech Republic
dc.contributor.institutionDepartment of Botany, University of BC, Vancouver, Canada
dc.contributor.institutionCanadian Institute for Advanced Research, Toronto, ON, Canada
dc.contributor.institutionWellcome Trust Sanger Institute, Hinxton, United Kingdom
dc.contributor.institutionInstitute of Microbiology, Czech Academy of Sciences, Třeboň, Czech Republic
kaust.personPain, Arnab
kaust.grant.numberFIC/2010/09
kaust.acknowledged.supportUnitBioscience Core Laboratory
dc.date.published-online2015-02-06
dc.date.published-print2015-05


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