Divergent mitochondrial respiratory chains in phototrophic relatives of apicomplexan parasites
Lai, De Hua
Otto, Thomas D.
Keeling, Patrick J.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Pathogen Genomics Laboratory
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AbstractFour respiratory complexes and ATP-synthase represent central functional units in mitochondria. In some mitochondria and derived anaerobic organelles, a few or all of these respiratory complexes have been lost during evolution. We show that the respiratory chain of Chromera velia, a phototrophic relative of parasitic apicomplexans, lacks complexes I and III, making it a uniquely reduced aerobic mitochondrion. In Chromera, putative lactate:cytochrome c oxidoreductases are predicted to transfer electrons from lactate to cytochrome c, rendering complex III unnecessary. The mitochondrial genome of Chromera has the smallest known protein-coding capacity of all mitochondria, encoding just cox1 and cox3 on heterogeneous linear molecules. In contrast, another photosynthetic relative of apicomplexans, Vitrella brassicaformis, retains the same set of genes as apicomplexans and dinoflagellates (cox1, cox3, and cob). © The Author 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.
SponsorsThe authors thank the KAUST Bioscience Core Laboratory personnel for sequencing Illumina libraries used in this project, Evgeny S. Gerasimov (Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow) and Martin Kolisko (University of British Columbia, Vancouver) for help with sequence analysis, Oldrich Benada (Institute of Microbiology, Prague) for help with electron microscopy, Anton Horvath (Comenius University, Bratislava), Dave Speijer (University of Amsterdam, Amsterdam), and Ivan Hrdy (Charles University, Prague) for useful comments. This work was supported by Czech Science Foundation grants P506/12/1522, 13-33039S (support for J.M.) and P501/12/G055 (support for A.T.) to M.O., project Algatech (CZ.1.05/2.1.00/03.0110) to M.O., FP7 agreement 316304 to M.O. and J.L., the KAUST award FIC/2010/09 to A.P., M.O. and J.L., and by a grant from the Canadian Institutes for Health Research to P.J.K. and J.L. is also supported by the Praemium Academiae, and P.F. by a grant of the Moravian-Silesian region (project MSK2013-DT1) and by Russian Foundation for Basic Research (project 14-04-31936). P.J.K. and J.L. are Senior Fellows and J.J. is a Global Scholar at the Canadian Institute for Advanced Research, and P.J.K. was supported by a Fellowship from the John Simon Guggenheim Foundation.
PublisherOxford University Press (OUP)
JournalMolecular Biology and Evolution
- The Organellar Genomes of Chromera and Vitrella, the Phototrophic Relatives of Apicomplexan Parasites.
- Authors: Oborník M, Lukeš J
- Issue date: 2015
- Chromera velia: The Missing Link in the Evolution of Parasitism.
- Authors: Weatherby K, Carter D
- Issue date: 2013
- Sterol Composition and Biosynthetic Genes of Vitrella brassicaformis, a Recently Discovered Chromerid: Comparison to Chromera velia and Phylogenetic Relationship with Apicomplexan Parasites.
- Authors: Khadka M, Salem M, Leblond JD
- Issue date: 2015 Nov-Dec
- Separation and identification of lipids in the photosynthetic cousins of Apicomplexa Chromera velia and Vitrella brassicaformis.
- Authors: Tomčala A, Kyselová V, Schneedorferová I, Opekarová I, Moos M, Urajová P, Kručinská J, Oborník M
- Issue date: 2017 Sep
- Alveolate mitochondrial metabolic evolution: dinoflagellates force reassessment of the role of parasitism as a driver of change in apicomplexans.
- Authors: Danne JC, Gornik SG, Macrae JI, McConville MJ, Waller RF
- Issue date: 2013 Jan