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dc.contributor.authorShahzad, K.
dc.contributor.authorRauf, M.
dc.contributor.authorEl-Zohry, Ahmed M.
dc.contributor.authorMalik, Z. A.
dc.contributor.authorHabib, I.
dc.contributor.authorAhmed, Z.
dc.contributor.authorMahmood, K.
dc.contributor.authorAli, R.
dc.contributor.authorMasmoudi, K.
dc.contributor.authorLemtiri-Chlieh, Fouad
dc.contributor.authorGehring, Christoph A
dc.contributor.authorBerkowitz, G. A.
dc.contributor.authorSaeed, N. A.
dc.date.accessioned2015-08-03T12:22:35Z
dc.date.available2015-08-03T12:22:35Z
dc.date.issued2015-01-29
dc.identifier.citationShahzad, K., Rauf, M., Ahmed, M., Malik, Z. A., Habib, I., Ahmed, Z., … Saeed, N. A. (2015). Functional characterisation of an intron retaining K+transporter of barley reveals intron-mediated alternate splicing. Plant Biology, 17(4), 840–851. doi:10.1111/plb.12290
dc.identifier.issn14358603
dc.identifier.pmid25631371
dc.identifier.doi10.1111/plb.12290
dc.identifier.urihttp://hdl.handle.net/10754/564001
dc.description.abstractIntron retention in transcripts and the presence of 5 and 3 splice sites within these introns mediate alternate splicing, which is widely observed in animals and plants. Here, functional characterisation of the K+ transporter, HvHKT2;1, with stably retained introns from barley (Hordeum vulgare) in yeast (Saccharomyces cerevisiae), and transcript profiling in yeast and transgenic tobacco (Nicotiana tabacum) is presented. Expression of intron-retaining HvHKT2;1 cDNA (HvHKT2;1-i) in trk1, trk2 yeast strain defective in K+ uptake restored growth in medium containing hygromycin in the presence of different concentrations of K+ and mediated hypersensitivity to Na+. HvHKT2;1-i produces multiple transcripts via alternate splicing of two regular introns and three exons in different compositions. HKT isoforms with retained introns and exon skipping variants were detected in relative expression analysis of (i) HvHKT2;1-i in barley under native conditions, (ii) in transgenic tobacco plants constitutively expressing HvHKT2;1-i, and (iii) in trk1, trk2 yeast expressing HvHKT2;1-i under control of an inducible promoter. Mixed proportions of three HKT transcripts: HvHKT2;1-e (first exon region), HvHKT2;1-i1 (first intron) and HvHKT2;1-i2 (second intron) were observed. The variation in transcript accumulation in response to changing K+ and Na+ concentrations was observed in both heterologous and plant systems. These findings suggest a link between intron-retaining transcripts and different splice variants to ion homeostasis, and their possible role in salt stress.
dc.publisherWiley
dc.subjectAlternate splicing
dc.subjectFunctional complementation
dc.subjectHvHKT2
dc.subjectIntron retention
dc.subjectTranscriptional gene regulation
dc.subjectTransporters and channels
dc.titleFunctional characterisation of an intron retaining K+ transporter of barley reveals intron-mediated alternate splicing
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentEnvironmental Science and Engineering Program
dc.contributor.departmentMolecular Signalling Group
dc.identifier.journalPlant Biology
dc.contributor.institutionNational Institute for Biotechnology and Genetic Engineering Faisalabad Pakistan
dc.contributor.institutionPakistan Institute of Engineering and Applied Sciences Nilore, Islamabad Pakistan
dc.contributor.institutionDepartment of Molecular and Cellular Biology University of Guelph Guelph, ON Canada
dc.contributor.institutionAgricultural Biotechnology Laboratory Department of Plant Science University of Connecticut Storrs, CT USA
dc.contributor.institutionInternational Centre for Biosaline Agriculture (ICBA) Dubai UAE
kaust.personLemtiri-Chlieh, Fouad
kaust.personGehring, Christoph A.
dc.date.published-online2015-01-29
dc.date.published-print2015-07


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