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    Hydrophilic interaction liquid chromatography method for measuring the composition of aquatic humic substances

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    Type
    Article
    Authors
    Wang, Renqi
    Gutierrez Garces, Leonardo
    Ng, Siuchoon
    Croue, Jean-Philippe
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Environmental Science and Engineering Program
    Water Desalination and Reuse Research Center (WDRC)
    Date
    2015-01
    Permanent link to this record
    http://hdl.handle.net/10754/563993
    
    Metadata
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    Abstract
    A hydrophilic interaction liquid chromatography (HILIC) method was developed to measure the composition of humic substances from river, reservoir, and treated wastewater based on their physicochemical properties. The current method fractionates the humic substances into four well-defined groups based on parallel analyses with a neutral and a cationic HILIC column, using mobile phases of varied compositions and pH. The results indicate that: (i) the proportion of carboxylic acids in the humic substances from terrestrial origins is less than half of that from treated wastewater (Jeddah, KSA), (ii) a higher content of basic compounds was observed in the humic substances from treated wastewater and Ribou Reservoir (Cholet, France) than in the sample from Loire River (France), (iii) a higher percentage of hydrophobic macromolecules were found in the humic substances from Loire River than in the other samples, and (iv) humic substances of treated wastewater contained less ionic neutral compounds (i.e., pKa 5-9) than the waters from terrestrial origins. The physicochemical property disparity amongst the compounds in each humic substances sample was also evaluated. The humic substances from the lightly humic Loire river displayed the highest disparity, whereas the highly humic Suwannee river (Georgia, USA) showed the most homogeneous humic substances.
    Citation
    Wang, R.-Q., Gutierrez, L., Choon, N. S., & Croué, J.-P. (2015). Hydrophilic interaction liquid chromatography method for measuring the composition of aquatic humic substances. Analytica Chimica Acta, 853, 608–616. doi:10.1016/j.aca.2014.09.026
    Publisher
    Elsevier BV
    Journal
    Analytica Chimica Acta
    DOI
    10.1016/j.aca.2014.09.026
    PubMed ID
    25467510
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.aca.2014.09.026
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC)

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