Hydrophilic interaction liquid chromatography method for measuring the composition of aquatic humic substances
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Environmental Science and Engineering Program
Water Desalination and Reuse Research Center (WDRC)
Permanent link to this recordhttp://hdl.handle.net/10754/563993
MetadataShow full item record
AbstractA hydrophilic interaction liquid chromatography (HILIC) method was developed to measure the composition of humic substances from river, reservoir, and treated wastewater based on their physicochemical properties. The current method fractionates the humic substances into four well-defined groups based on parallel analyses with a neutral and a cationic HILIC column, using mobile phases of varied compositions and pH. The results indicate that: (i) the proportion of carboxylic acids in the humic substances from terrestrial origins is less than half of that from treated wastewater (Jeddah, KSA), (ii) a higher content of basic compounds was observed in the humic substances from treated wastewater and Ribou Reservoir (Cholet, France) than in the sample from Loire River (France), (iii) a higher percentage of hydrophobic macromolecules were found in the humic substances from Loire River than in the other samples, and (iv) humic substances of treated wastewater contained less ionic neutral compounds (i.e., pKa 5-9) than the waters from terrestrial origins. The physicochemical property disparity amongst the compounds in each humic substances sample was also evaluated. The humic substances from the lightly humic Loire river displayed the highest disparity, whereas the highly humic Suwannee river (Georgia, USA) showed the most homogeneous humic substances.
JournalAnalytica Chimica Acta
- TiO2 nanoparticles aggregation and disaggregation in presence of alginate and Suwannee River humic acids. pH and concentration effects on nanoparticle stability.
- Authors: Loosli F, Le Coustumer P, Stoll S
- Issue date: 2013 Oct 15
- Assessing the trihalomethane formation potential of aquatic fulvic and humic acids fractionated using thin-layer chromatography.
- Authors: Eish MY, Wells MJ
- Issue date: 2006 May 26
- Monitoring changes in the structure and properties of humic substances following ozonation using UV-Vis, FTIR and (1)H NMR techniques.
- Authors: Rodríguez FJ, Schlenger P, García-Valverde M
- Issue date: 2016 Jan 15
- Coating of AFM probes with aquatic humic and non-humic NOM to study their adhesion properties.
- Authors: Aubry C, Gutierrez L, Croue JP
- Issue date: 2013 Jun 1
- Spectral and temporal luminescent properties of Eu(III) in humic substance solutions from different origins.
- Authors: Brevet J, Claret F, Reiller PE
- Issue date: 2009 Oct 1