Show simple item record

dc.contributor.authorAriel, Federico D.
dc.contributor.authorJégu, Teddy
dc.contributor.authorLatrasse, David
dc.contributor.authorRomero-Barrios, Natali
dc.contributor.authorChrist, Aurélie
dc.contributor.authorBenhamed, Moussa
dc.contributor.authorCrespi, Martín D.
dc.date.accessioned2015-08-03T12:06:16Z
dc.date.available2015-08-03T12:06:16Z
dc.date.issued2014-08
dc.identifier.issn10972765
dc.identifier.pmid25018019
dc.identifier.doi10.1016/j.molcel.2014.06.011
dc.identifier.urihttp://hdl.handle.net/10754/563684
dc.description.abstractThe eukaryotic epigenome is shaped by the genome topology in three-dimensional space. Dynamic reversible variations in this epigenome structure directly influence the transcriptional responses to developmental cues. Here, we show that the Arabidopsis long intergenic noncoding RNA (lincRNA) APOLO is transcribed by RNA polymerases II and V in response to auxin, a phytohormone controlling numerous facets of plant development. This dual APOLO transcription regulates the formation of a chromatin loop encompassing the promoter of its neighboring gene PID, a key regulator of polar auxin transport. Altering APOLO expression affects chromatin loop formation, whereas RNA-dependent DNA methylation, active DNA demethylation, and Polycomb complexes control loop dynamics. This dynamic chromatin topology determines PID expression patterns. Hence, the dual transcription of a lincRNA influences local chromatin topology and directs dynamic auxin-controlled developmental outputs on neighboring genes. This mechanism likely underscores the adaptive success of plants in diverse environments and may be widespread in eukaryotes. © 2014 Elsevier Inc.
dc.description.sponsorshipWe thank Herve Vaucheret for rdd seeds, Francois Roudier for clf and swn seeds, and both of them for constructive discussions, as well as Allison Mallory for careful reading of the manuscript. We also thank Craig Pikaard for NRPE1-FLAG-transformed seeds and Koji Goto for LHP1-GFP-transformed seeds. F.A. is an EMBO postdoc fellow; N.R.-B. and T.J. are Paris-Sud University PhD fellows. This work was funded by LABEX Saclay Plant Sciences and the France-Brazilian COFECUB Exchange program.
dc.publisherElsevier BV
dc.titleNoncoding transcription by alternative rna polymerases dynamically regulates an auxin-driven chromatin loop
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentPlant Science Program
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentBioscience Program
dc.contributor.departmentChromatin and development Research Group
dc.identifier.journalMolecular Cell
dc.contributor.institutionCNRS, Institut des Sciences du Végétal, Saclay Plant Sciences, 91198 Gif-sur-Yvette and Universite Paris Diderot-Paris 7, 75013 Paris, France
dc.contributor.institutionInstitut de Biologie des Plantes, UMR8618 Université Paris-Sud XI, SPS Saclay Plant Sciences, 91405 Orsay, France
kaust.personBenhamed, Moussa


This item appears in the following Collection(s)

Show simple item record