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    Microprofiles of oxygen, redox potential, and pH, and microbial fermentation products in the highly alkaline gut of the saprophagous larva of Penthetria holosericea (Diptera: Bibionidae)

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    Type
    Article
    Authors
    Šustr, Vladimír
    Stingl, Ulrich cc
    Brune, Andreas
    KAUST Department
    Red Sea Research Center (RSRC)
    Biological and Environmental Sciences and Engineering (BESE) Division
    Marine Science Program
    Marine Microbial Ecology Research Group
    Date
    2014-08
    Permanent link to this record
    http://hdl.handle.net/10754/563675
    
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    Abstract
    The saprophagous larvae of bibionid flies harbor bacteria in their alkaline intestinal tracts, but little is known about the contribution of the gut microbiota to the digestion of their recalcitrant diet. In this study, we measured oxygen and hydrogen partial pressure, redox potential and pH in the midgut, gastric caeca and hindgut of larvae of the bibionid fly Penthetria holosericea with Clark-type O2 and H2 microsensors, platinum redox microelectrodes, and LIX-type pH microelectrodes. The center of the midgut lumen was anoxic, whereas gastric caeca and hindgut were hypoxic. However, redox potential profiles indicated oxidizing conditions throughout the gut, with lowest values in the midgut (+20 to +60mV). Hydrogen production was not detected. The midgut was extremely alkaline (pH around 11), whereas hindgut and gastric caeca were neutral to slightly alkaline. While HPLC analysis showed high concentrations of glucose in the midgut (15mM) and gastric caeca (27mM), the concentrations of microbial fermentation products such as lactate (2-4mM), acetate (<1mM) and succinate (<0.5mM) were low in all gut regions, suggesting that the contribution of microorganisms to the digestive process, particularly in the alkaline midgut, is only of minor importance. We conclude that the digestive strategy of the saprophytic larva of P. holosericea, which feeds selectively on decomposed leaves and its own microbe-rich faeces, differs fundamentally from those of detritivorous and humivorous insects, which host a highly active, fermentative microbiota in their alkaline midgut or hindgut compartments. © 2014 Elsevier Ltd.
    Citation
    Šustr, V., Stingl, U., & Brune, A. (2014). Microprofiles of oxygen, redox potential, and pH, and microbial fermentation products in the highly alkaline gut of the saprophagous larva of Penthetria holosericea (Diptera: Bibionidae). Journal of Insect Physiology, 67, 64–69. doi:10.1016/j.jinsphys.2014.06.007
    Publisher
    Elsevier BV
    Journal
    Journal of Insect Physiology
    DOI
    10.1016/j.jinsphys.2014.06.007
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.jinsphys.2014.06.007
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Red Sea Research Center (RSRC); Marine Science Program

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