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dc.contributor.authorMalara, Natalia
dc.contributor.authorColuccio, Maria Laura
dc.contributor.authorLimongi, Tania
dc.contributor.authorAsande, Monica
dc.contributor.authorTrunzo, Valentina
dc.contributor.authorCojoc, Gheorghe
dc.contributor.authorRaso, Cinzia
dc.contributor.authorCandeloro, Patrizio
dc.contributor.authorPerozziello, Gerardo
dc.contributor.authorRaimondo, Raffaella
dc.contributor.authorDe Vitis, Stefania
dc.contributor.authorRoveda, Laura
dc.contributor.authorRenne, Maria
dc.contributor.authorPrati, Ubaldo
dc.contributor.authorMollace, Vincenzo
dc.contributor.authorDi Fabrizio, Enzo M.
dc.date.accessioned2015-08-03T12:04:42Z
dc.date.available2015-08-03T12:04:42Z
dc.date.issued2014-07-14
dc.identifier.issn16136810
dc.identifier.doi10.1002/smll.201400498
dc.identifier.doi10.1002/smll.201470135
dc.identifier.urihttp://hdl.handle.net/10754/563624
dc.description.abstractAlthough the detection of methylated cell free DNA represents one of the most promising approaches for relapse risk assessment in cancer patients, the low concentration of cell-free circulating DNA constitutes the biggest obstacle in the development of DNA methylation-based biomarkers from blood. This paper describes a method for the measurement of genomic methylation content directly on circulating tumor cells (CTC), which could be used to deceive the aforementioned problem. Since CTC are disease related blood-based biomarkers, they result essential to monitor tumor's stadiation, therapy, and early relapsing lesions. Within surface's bio-functionalization and cell's isolation procedure standardization, the presented approach reveals a singular ability to detect high 5-methylcytosine CTC-subset content in the whole CTC compound, by choosing folic acid (FA) as transducer molecule. Sensitivity and specificity, calculated for FA functionalized surface (FA-surface), result respectively on about 83% and 60%. FA-surface, allowing the detection and characterization of early metastatic dissemination, provides a unique advance in the comprehension of tumors progression and dissemination confirming the presence of CTC and its association with high risk of relapse. This functionalized surface identifying and quantifying high 5-methylcytosine CTC-subset content into the patient's blood lead significant progress in cancer risk assessment, also providing a novel therapeutic strategy.© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
dc.description.sponsorshipThis work was partially supported by the Grants from the PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti" project (code: PON01_02782), the Interregional Research Centre for Food Safety & Health (IRC_FSC) project (cod. PON a3-00359) granted to the Department of Health Science of the University Magna Graecia of Catanzaro, the FIRB "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" project (cod. RBPR05JH2P_010, CUP B41J09000110005) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro and the "Fondo Sociale Europeo - POR Calabria FSE 2007/2013" Program. The authors thank R. Giammaria for revising the English text.
dc.publisherWiley-VCH Verlag
dc.subjectFolic acid
dc.subjectFolic acid receptors
dc.subjectFunctionalized surfaces
dc.subjectHyper-methylated circulating tumor cells
dc.titleFolic acid functionalized surface highlights 5-methylcytosine-genomic content within circulating tumor cells
dc.typeArticle
dc.contributor.departmentMaterial Science and Engineering Program
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalSmall
dc.contributor.institutionItalian Institute of TechnologyArnesano, Lecce, Italy
dc.contributor.institutionBIONEM, University of Magna Graecia, Campus S. Venuta, Viale EuropaCatanzaro, Italy
dc.contributor.institutionFaculty of Pharmacy, University of Magna Graecia, Campus Salvatore Venuta, Viale EuropaCatanzaro, Italy
dc.contributor.institutionMax Planck Institute of Molecular, Cell Biology and Genetics, Pfotenhauerstrasse 108Dresden, Germany
dc.contributor.institutionSystems Biology Ireland, Conway Institute UCDDublin, Belfield, Ireland
dc.contributor.institutionOncologic Surgery, Cancer Centre of Excellence Tommaso Campanella Foundation, Viale EuropaCatanzaro, Italy
dc.contributor.institutionInterregional Research Center on Food Safety and Health (IRC-FSH), Department of Health Sciences, University Magna Graecia of CatanzaroCatanzaro, Italy
kaust.personLimongi, Tania
kaust.personDi Fabrizio, Enzo M.
dc.date.published-online2014-07-14
dc.date.published-print2014-07


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