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    Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

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    Type
    Article
    Authors
    De Vitis, Stefania
    Matarise, Giuseppina
    Pardeo, Francesca
    Catalano, Rossella
    Malara, Natalia Maria
    Trunzo, Valentina
    Tallerico, Rossana
    Gentile, Francesco T.
    Candeloro, Patrizio cc
    Coluccio, Maria Laura
    Massaro, Alessandro S.
    Viglietto, Giuseppe
    Carbone, Ennio
    Kutter, Jörg Peter
    Perozziello, Gerardo
    Di Fabrizio, Enzo M. cc
    KAUST Department
    Material Science and Engineering Program
    Physical Science and Engineering (PSE) Division
    Date
    2014-07
    Permanent link to this record
    http://hdl.handle.net/10754/563614
    
    Metadata
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    Abstract
    The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs. © 2014 Elsevier B.V. All rights reserved.
    Citation
    De Vitis, S., Matarise, G., Pardeo, F., Catalano, R., Malara, N., Trunzo, V., … Di Fabrizio, E. (2014). Isolation of cancer cells by “in situ” microfluidic biofunctionalization protocols. Microelectronic Engineering, 124, 76–80. doi:10.1016/j.mee.2014.04.013
    Sponsors
    This work was partially supported by the project PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti" (code: PON01_02782), the European project EUROMBR (grant agreement n.608104), the project for Young Researchers financed from the Italian Ministry of Health (CUP J65C13001350001, project n. GR-2010-2311677) and the project FIRB "ReteNazionale di Ricerca sulle Nanoscienze ItalNanoNet" (code: RBPR05JH2P_010, CUP B41J09000110005) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro.
    Publisher
    Elsevier BV
    Journal
    Microelectronic Engineering
    DOI
    10.1016/j.mee.2014.04.013
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.mee.2014.04.013
    Scopus Count
    Collections
    Articles; Physical Science and Engineering (PSE) Division; Material Science and Engineering Program

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