Quantitative proteomics reveals differential biological processes in healthy neonatal cord neutrophils and adult neutrophils
KAUST DepartmentBioscience Core Lab
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AbstractNeonatal neutrophils are characterized by the immaturity of bactericidal mechanisms that contributes largely to neonatal mortality. However, underlying molecular mechanism associated with the immaturity remains incompletely understood. In this study, we performed comparative proteomic analysis on neonatal neutrophils derived from human cord blood and adult peripheral neutrophils. A total of 1332 proteins were identified and quantified, and 127 proteins were characterized as differentially expressed between adult and cord neutrophils. The differentially expressed proteins are mapped in KEGG pathways into five clusters and indicated impaired functions of neonatal neutrophils in proteasome, lysosome, phagosome, and leukocyte transendothelial migration. In particular, many proteins associated with NETosis, a critical mechanism for antimicrobial process and auto-clearance, were also found to be downregulated in cord neutrophils. This study represents a first comparative proteome profiling of neonatal and adult neutrophils, and provides a global view of differentially expressed proteome for enhancing our understanding of their various functional difference. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
SponsorsWe thank Jian Li and Dr Juan Li of the Flow Cytometry Unit in the Center for Stem Cell Research and Application, HUST, P.R China for their assistance in cell purity identification, and Dr. Jingqiong Hu for her help in cord blood collection. This study was supported by a grant from National Natural Science Foundation, P.R China (NNSF/30971112/2010).