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dc.contributor.authorJimenez Infante, Francy M.
dc.contributor.authorNgugi, David
dc.contributor.authorAlam, Intikhab
dc.contributor.authorRashid, Mamoon
dc.contributor.authorBa Alawi, Wail
dc.contributor.authorKamau, Allan
dc.contributor.authorBajic, Vladimir B.
dc.contributor.authorStingl, Ulrich
dc.date.accessioned2015-08-03T11:54:22Z
dc.date.available2015-08-03T11:54:22Z
dc.date.issued2014-05-22
dc.identifier.citationJimenez-Infante, F., Ngugi, D. K., Alam, I., Rashid, M., Baalawi, W., Kamau, A. A., … Stingl, U. (2014). Genomic differentiation among two strains of the PS1 clade isolated from geographically separated marine habitats. FEMS Microbiology Ecology, 89(1), 181–197. doi:10.1111/1574-6941.12348
dc.identifier.issn01686496
dc.identifier.pmid24785133
dc.identifier.doi10.1111/1574-6941.12348
dc.identifier.urihttp://hdl.handle.net/10754/563555
dc.description.abstractUsing dilution-to-extinction cultivation, we isolated a strain affiliated with the PS1 clade from surface waters of the Red Sea. Strain RS24 represents the second isolate of this group of marine Alphaproteobacteria after IMCC14465 that was isolated from the East (Japan) Sea. The PS1 clade is a sister group to the OCS116 clade, together forming a putatively novel order closely related to Rhizobiales. While most genomic features and most of the genetic content are conserved between RS24 and IMCC14465, their average nucleotide identity (ANI) is < 81%, suggesting two distinct species of the PS1 clade. Next to encoding two different variants of proteorhodopsin genes, they also harbor several unique genomic islands that contain genes related to degradation of aromatic compounds in IMCC14465 and in polymer degradation in RS24, possibly reflecting the physicochemical differences in the environment they were isolated from. No clear differences in abundance of the genomic content of either strain could be found in fragment recruitment analyses using different metagenomic datasets, in which both genomes were detectable albeit as minor part of the communities. The comparative genomic analysis of both isolates of the PS1 clade and the fragment recruitment analysis provide first insights into the ecology of this group. © 2014 Federation of European Microbiological Societies.
dc.publisherOxford University Press (OUP)
dc.subjectAlphaproteobacteria
dc.subjectDilution-to-extinction
dc.subjectOCS116 clade
dc.subjectProteorhodopsin
dc.subjectPS1 clade
dc.subjectRed Sea
dc.titleGenomic differentiation among two strains of the PS1 clade isolated from geographically separated marine habitats
dc.typeArticle
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentApplied Mathematics and Computational Science Program
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentMarine Science Program
dc.contributor.departmentMarine Microbial Ecology Research Group
dc.contributor.departmentComputer Science Program
dc.identifier.journalFEMS Microbiology Ecology
kaust.personNgugi, David
kaust.personAlam, Intikhab
kaust.personRashid, Mamoon
kaust.personBajic, Vladimir B.
kaust.personStingl, Ulrich
kaust.personJimenez Infante, Francy M.
kaust.personBa Alawi, Wail
kaust.personKamau, Allan
dc.relation.issupplementedbybioproject:PRJNA217824
display.relations<b>Is Supplemented By:</b><br/> <ul><li><i>[Bioproject]</i> <br/> Title: Candidatus Micropelagos thuwalensis strain:RS24 Genome sequencing and assemblyPublication Date: 2013-09-20. bioproject: <a href="https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA217824" >PRJNA217824</a> Handle: <a href="http://hdl.handle.net/10754/666427" >10754/666427</a></a></li></ul>
dc.date.published-online2014-05-22
dc.date.published-print2014-07


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