The effect of cerium valence states at cerium oxide nanoparticle surfaces on cell proliferation
KAUST DepartmentKAUST Solar Center (KSC)
Material Science and Engineering Program
Materials for Energy Conversion and Storage (MECS) Lab
Physical Science and Engineering (PSE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/563533
MetadataShow full item record
AbstractUnderstanding and controlling cell proliferation on biomaterial surfaces is critical for scaffold/artificial-niche design in tissue engineering. The mechanism by which underlying integrin ligates with functionalized biomaterials to induce cell proliferation is still not completely understood. In this study, poly-l-lactide (PL) scaffold surfaces were functionalized using layers of cerium oxide nanoparticles (CNPs), which have recently attracted attention for use in therapeutic application due to their catalytic ability of Ce4+ and Ce3+ sites. To isolate the influence of Ce valance states of CNPs on cell proliferation, human mesenchymal stem cells (hMSCs) and osteoblast-like cells (MG63) were cultured on the PL/CNP surfaces with dominant Ce4+ and Ce3+ regions. Despite cell type (hMSCs and MG63 cells), different surface features of Ce4+ and Ce3+ regions clearly promoted and inhibited cell spreading, migration and adhesion behavior, resulting in rapid and slow cell proliferation, respectively. Cell proliferation results of various modified CNPs with different surface charge and hydrophobicity/hydrophilicity, indicate that Ce valence states closely correlated with the specific cell morphologies and cell-material interactions that trigger cell proliferation. This finding suggests that the cell-material interactions, which influence cell proliferation, may be controlled by introduction of metal elements with different valence states onto the biomaterial surface. © 2014 Elsevier Ltd.
CitationNaganuma, T., & Traversa, E. (2014). The effect of cerium valence states at cerium oxide nanoparticle surfaces on cell proliferation. Biomaterials, 35(15), 4441–4453. doi:10.1016/j.biomaterials.2014.01.074
SponsorsThis work was supported, in part, by the World Premier International (WPI) Research Center Initiative Program. Technical support by the Bio-Organic Materials Facility in the Nanotechnology Innovation Station, NIMS, Japan is acknowledged. The authors thank Ms. H. Morita and Dr. X. LI for technical support and suggestions about biological experiments, Ms. S. Kajiwara for technical support of confocal microscope observations (Nanotechnology Innovation Station, NIMS, Japan). We would like to thank Prof. T. Aoyagi (International Center for Materials Nanoarchitectonics, NIMS, Japan) and Dr. L Ghibelli (University of Rome Tor Vergata) for invaluable discussions.
- Various physicochemical and surface properties controlling the bioactivity of cerium oxide nanoparticles.
- Authors: Chen BH, Stephen Inbaraj B
- Issue date: 2018 Nov
- Physicochemical and biological interactions between cerium oxide nanoparticles and a 1,8-naphthalimide derivative.
- Authors: Pulido-Reyes G, Martín E, Gu Coronado JL, Leganes F, Rosal R, Fernández-Piñas F
- Issue date: 2017 Jul
- Air, aqueous and thermal stabilities of Ce3+ ions in cerium oxide nanoparticle layers with substrates.
- Authors: Naganuma T, Traversa E
- Issue date: 2014 Jun 21
- Cerium Oxide Nanoparticle Modified Scaffold Interface Enhances Vascularization of Bone Grafts by Activating Calcium Channel of Mesenchymal Stem Cells.
- Authors: Xiang J, Li J, He J, Tang X, Dou C, Cao Z, Yu B, Zhao C, Kang F, Yang L, Dong S, Yang X
- Issue date: 2016 Feb
- Synthesis, physico-chemical characterization, and antioxidant effect of PEGylated cerium oxide nanoparticles.
- Authors: Xue Y, Balmuri SR, Patel A, Sant V, Sant S
- Issue date: 2018 Apr