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    On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7

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    Type
    Article
    Authors
    Bruno, Mark
    Hofmann, Manuel
    Vermathen, Martina
    Alder, Adrian
    Beyer, Peter D.
    Al-Babili, Salim cc
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Bioscience Program
    Center for Desert Agriculture
    Plant Science
    Plant Science Program
    Date
    2014-03-28
    Online Publication Date
    2014-03-28
    Print Publication Date
    2014-05-02
    Permanent link to this record
    http://hdl.handle.net/10754/563521
    
    Metadata
    Show full item record
    Abstract
    Strigolactones are phytohormones synthesized from carotenoids via a stereospecific pathway involving the carotenoid cleavage dioxygenases 7 (CCD7) and 8. CCD7 cleaves 9-cis-β-carotene to form a supposedly 9-cis-configured β-apo-10′-carotenal. CCD8 converts this intermediate through a combination of yet undetermined reactions into the strigolactone-like compound carlactone. Here, we investigated the substrate and stereo-specificity of the Arabidopsis and pea CCD7 and determined the stereo-configuration of the β-apo-10′-carotenal intermediate by using Nuclear Magnetic Resonance Spectroscopy. Our data unequivocally demonstrate the 9-cis-configuration of the intermediate. Both CCD7s cleave different 9-cis-carotenoids, yielding hydroxylated 9-cis-apo-10′-carotenals that may lead to hydroxylated carlactones, but show highest affinity for 9-cis-β-carotene. © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
    Citation
    Bruno, M., Hofmann, M., Vermathen, M., Alder, A., Beyer, P., & Al-Babili, S. (2014). On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7. FEBS Letters, 588(9), 1802–1807. doi:10.1016/j.febslet.2014.03.041
    Sponsors
    This work was supported by the German Research Foundation (DFG) Grant AL 892/1-4 and by the EU (METAPRO; FP7 KBBE-2009-3-1-01).
    Publisher
    Wiley
    Journal
    FEBS Letters
    DOI
    10.1016/j.febslet.2014.03.041
    PubMed ID
    24685691
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.febslet.2014.03.041
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Plant Science Program; Center for Desert Agriculture

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