On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7
Type
ArticleKAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionBioscience Program
Center for Desert Agriculture
Plant Science
Plant Science Program
Date
2014-03-28Online Publication Date
2014-03-28Print Publication Date
2014-05-02Permanent link to this record
http://hdl.handle.net/10754/563521
Metadata
Show full item recordAbstract
Strigolactones are phytohormones synthesized from carotenoids via a stereospecific pathway involving the carotenoid cleavage dioxygenases 7 (CCD7) and 8. CCD7 cleaves 9-cis-β-carotene to form a supposedly 9-cis-configured β-apo-10′-carotenal. CCD8 converts this intermediate through a combination of yet undetermined reactions into the strigolactone-like compound carlactone. Here, we investigated the substrate and stereo-specificity of the Arabidopsis and pea CCD7 and determined the stereo-configuration of the β-apo-10′-carotenal intermediate by using Nuclear Magnetic Resonance Spectroscopy. Our data unequivocally demonstrate the 9-cis-configuration of the intermediate. Both CCD7s cleave different 9-cis-carotenoids, yielding hydroxylated 9-cis-apo-10′-carotenals that may lead to hydroxylated carlactones, but show highest affinity for 9-cis-β-carotene. © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.Citation
Bruno, M., Hofmann, M., Vermathen, M., Alder, A., Beyer, P., & Al-Babili, S. (2014). On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7. FEBS Letters, 588(9), 1802–1807. doi:10.1016/j.febslet.2014.03.041Sponsors
This work was supported by the German Research Foundation (DFG) Grant AL 892/1-4 and by the EU (METAPRO; FP7 KBBE-2009-3-1-01).Publisher
WileyJournal
FEBS LettersPubMed ID
24685691ae974a485f413a2113503eed53cd6c53
10.1016/j.febslet.2014.03.041
Scopus Count
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