Pyrosequencing Reveals the Microbial Communities in the Red Sea Sponge Carteriospongia foliascens and Their Impressive Shifts in Abnormal Tissues
Batang, Zenon B.
Al-Suwailem, Abdulaziz M.
Lafi, Feras Fawzi
Bajic, Vladimir B.
KAUST DepartmentComputational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Coastal and Marine Resources Core Lab
Desert Agriculture Initiative
Biological and Environmental Sciences and Engineering (BESE) Division
Applied Mathematics and Computational Science Program
Permanent link to this recordhttp://hdl.handle.net/10754/563510
MetadataShow full item record
AbstractAbnormality and disease in sponges have been widely reported, yet how sponge-associated microbes respond correspondingly remains inconclusive. Here, individuals of the sponge Carteriospongia foliascens under abnormal status were collected from the Rabigh Bay along the Red Sea coast. Microbial communities in both healthy and abnormal sponge tissues and adjacent seawater were compared to check the influences of these abnormalities on sponge-associated microbes. In healthy tissues, we revealed low microbial diversity with less than 100 operational taxonomic units (OTUs) per sample. Cyanobacteria, affiliated mainly with the sponge-specific species “Candidatus Synechococcus spongiarum,” were the dominant bacteria, followed by Bacteroidetes and Proteobacteria. Intraspecies dynamics of microbial communities in healthy tissues were observed among sponge individuals, and potential anoxygenic phototrophic bacteria were found. In comparison with healthy tissues and the adjacent seawater, abnormal tissues showed dramatic increase in microbial diversity and decrease in the abundance of sponge-specific microbial clusters. The dominated cyanobacterial species Candidatus Synechococcus spongiarum decreased and shifted to unspecific cyanobacterial clades. OTUs that showed high similarity to sequences derived from diseased corals, such as Leptolyngbya sp., were found to be abundant in abnormal tissues. Heterotrophic Planctomycetes were also specifically enriched in abnormal tissues. Overall, we revealed the microbial communities of the cyanobacteria-rich sponge, C. foliascens, and their impressive shifts under abnormality.
SponsorsThe authors thank WP Zhang and G Zhang from Hong Kong University of Science and Technology (HKUST) and the technical team from King Abdullah University of Science and Technology (KAUST) for technical help during sample collection. The authors also thank Professor Rob von Soest, Zoological Museum, University of Amsterdam, for identification of sponges. This study was supported by a grant (U13012056) from the National Science Foundation of China, a grant from China Ocean Mineral Resource Research and Development Association (COMRRDA12SC02), and an award (SA-C0040/UK-C0016) granted to P.Y. Qian from the King Abdullah University of Science and Technology. FFL is supported by the KAUST Special Collaborative Partnership grant. VBB is supported by the KAUST Base Research Funds.
PublisherSpringer Science + Business Media
CollectionsArticles; Biological and Environmental Sciences and Engineering (BESE) Division; Coastal and Marine Resources Core Lab; Applied Mathematics and Computational Science Program; Computational Bioscience Research Center (CBRC); Desert Agriculture Initiative; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
- Specificity and transcriptional activity of microbiota associated with low and high microbial abundance sponges from the Red Sea.
- Authors: Moitinho-Silva L, Bayer K, Cannistraci CV, Giles EC, Ryu T, Seridi L, Ravasi T, Hentschel U
- Issue date: 2014 Mar
- Pyrosequencing reveals highly diverse and species-specific microbial communities in sponges from the Red Sea.
- Authors: Lee OO, Wang Y, Yang J, Lafi FF, Al-Suwailem A, Qian PY
- Issue date: 2011 Apr
- Pyrosequencing reveals diverse and distinct sponge-specific microbial communities in sponges from a single geographical location in Irish waters.
- Authors: Jackson SA, Kennedy J, Morrissey JP, O'Gara F, Dobson AD
- Issue date: 2012 Jul
- First insights into the microbiome of a carnivorous sponge.
- Authors: Dupont S, Corre E, Li Y, Vacelet J, Bourguet-Kondracki ML
- Issue date: 2013 Dec
- Cultivable bacterial community from South China Sea sponge as revealed by DGGE fingerprinting and 16S rDNA phylogenetic analysis.
- Authors: Li Z, He L, Miao X
- Issue date: 2007 Dec