Show simple item record

dc.contributor.authorViržonis, Darius
dc.contributor.authorVanagas, Gailius
dc.contributor.authorRamanavičiene, Almira
dc.contributor.authorMakaraviciute, Asta
dc.contributor.authorBarauskas, Dovydas
dc.contributor.authorRamanavičius, Arũnas
dc.contributor.authorWen, Weijia
dc.contributor.authorKodzius, Rimantas
dc.date.accessioned2015-08-03T11:52:50Z
dc.date.available2015-08-03T11:52:50Z
dc.date.issued2014-04-08
dc.identifier.citationVirzonis, D., Vanagas, G., Ramanaviciene, A., Makaraviciute, A., Barauskas, D., Ramanavicius, A., … Kodzius, R. (2014). Resonant gravimetric immunosensing based on capacitive micromachined ultrasound transducers. Microchimica Acta, 181(13-14), 1749–1757. doi:10.1007/s00604-014-1244-3
dc.identifier.issn00263672
dc.identifier.doi10.1007/s00604-014-1244-3
dc.identifier.urihttp://hdl.handle.net/10754/563494
dc.description.abstractHigh-frequency (40 MHz) and low-frequency (7 MHz) capacitive micromachined ultrasound transducers (CMUT) were fabricated and tested for use in gravimetric detection of biomolecules. The low-frequency CMUT sensors have a gold-coated surface, while the high-frequency sensors have a silicon nitride surface. Both surfaces were functionalized with bovine leukemia virus antigen gp51 acting as the antigen. On addition of an a specific antibody labeled with horseradish peroxidase (HRP), the antigen/antibody complex is formed on the surface and quantified by HRP-catalyzed oxidation of tetramethylbenzidine. It has been found that a considerably smaller quantity of immuno complex is formed on the high frequency sensor surface. In parallel, the loading of the surface of the CMUT was determined via resonance frequency and electromechanical resistance readings. Following the formation of the immuno complexes, the resonance frequencies of the low-frequency and high-frequency sensors decrease by up to 420 and 440 kHz, respectively. Finite element analysis reveals that the loading of the (gold-coated) low frequency sensors is several times larger than that on high frequency sensors. The formation of the protein film with pronounced elasticity and stress on the gold surface case is discussed. We also discuss the adoption of this method for the detection of DNA using a hybridization assay following polymerase chain reaction.
dc.description.sponsorshipThis research is funded by a grant (No. MIP-059/2012) from the Research Council of Lithuania.
dc.publisherSpringer Nature
dc.subjectCapacitive micromachined ultrasound transducers
dc.subjectDNA hybridization
dc.subjectGravimetric sensors
dc.subjectImmunosensing
dc.subjectLabel-free biomolecule detection
dc.subjectSensor arrays
dc.titleResonant gravimetric immunosensing based on capacitive micromachined ultrasound transducers
dc.typeArticle
dc.contributor.departmentKAUST-HKUST Micro/Nanofluidic Joint Laboratory
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentEnvironmental Science and Engineering Program
dc.identifier.journalMicrochimica Acta
dc.contributor.institutionDepartment of Technologies, Daukanto 12Panevezys, Lithuania
dc.contributor.institutionNanoTechnas – Center of Nanotechnology and Materials Science, Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24Vilnius, Lithuania
dc.contributor.institutionDepartment of Physical Chemistry, Vilnius University, Naugarduko 24Vilnius, Lithuania
dc.contributor.institutionLaboratory of BioNanoTechnology, Department of Material Science and Electrical Engineering, Semiconductor Physics Institute, State Research Institute Center for Physical and Technological Sciences, A. Gostauto 11Vilnius, Lithuania
kaust.personKodzius, Rimantas
dc.date.published-online2014-04-08
dc.date.published-print2014-10


This item appears in the following Collection(s)

Show simple item record