Resonant gravimetric immunosensing based on capacitive micromachined ultrasound transducers
Type
ArticleAuthors
Viržonis, DariusVanagas, Gailius
Ramanavičiene, Almira
Makaraviciute, Asta
Barauskas, Dovydas
Ramanavičius, Arũnas
Wen, Weijia
Kodzius, Rimantas

KAUST Department
KAUST-HKUST Micro/Nanofluidic Joint LaboratoryBiological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Environmental Science and Engineering Program
Date
2014-04-08Online Publication Date
2014-04-08Print Publication Date
2014-10Permanent link to this record
http://hdl.handle.net/10754/563494
Metadata
Show full item recordAbstract
High-frequency (40 MHz) and low-frequency (7 MHz) capacitive micromachined ultrasound transducers (CMUT) were fabricated and tested for use in gravimetric detection of biomolecules. The low-frequency CMUT sensors have a gold-coated surface, while the high-frequency sensors have a silicon nitride surface. Both surfaces were functionalized with bovine leukemia virus antigen gp51 acting as the antigen. On addition of an a specific antibody labeled with horseradish peroxidase (HRP), the antigen/antibody complex is formed on the surface and quantified by HRP-catalyzed oxidation of tetramethylbenzidine. It has been found that a considerably smaller quantity of immuno complex is formed on the high frequency sensor surface. In parallel, the loading of the surface of the CMUT was determined via resonance frequency and electromechanical resistance readings. Following the formation of the immuno complexes, the resonance frequencies of the low-frequency and high-frequency sensors decrease by up to 420 and 440 kHz, respectively. Finite element analysis reveals that the loading of the (gold-coated) low frequency sensors is several times larger than that on high frequency sensors. The formation of the protein film with pronounced elasticity and stress on the gold surface case is discussed. We also discuss the adoption of this method for the detection of DNA using a hybridization assay following polymerase chain reaction.Citation
Virzonis, D., Vanagas, G., Ramanaviciene, A., Makaraviciute, A., Barauskas, D., Ramanavicius, A., … Kodzius, R. (2014). Resonant gravimetric immunosensing based on capacitive micromachined ultrasound transducers. Microchimica Acta, 181(13-14), 1749–1757. doi:10.1007/s00604-014-1244-3Sponsors
This research is funded by a grant (No. MIP-059/2012) from the Research Council of Lithuania.Publisher
Springer NatureJournal
Microchimica Actaae974a485f413a2113503eed53cd6c53
10.1007/s00604-014-1244-3