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    Resonant gravimetric immunosensing based on capacitive micromachined ultrasound transducers

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    Type
    Article
    Authors
    Viržonis, Darius
    Vanagas, Gailius
    Ramanavičiene, Almira
    Makaraviciute, Asta
    Barauskas, Dovydas
    Ramanavičius, Arũnas
    Wen, Weijia
    Kodzius, Rimantas cc
    KAUST Department
    KAUST-HKUST Micro/Nanofluidic Joint Laboratory
    Biological and Environmental Sciences and Engineering (BESE) Division
    Computational Bioscience Research Center (CBRC)
    Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
    Environmental Science and Engineering Program
    Date
    2014-04-08
    Online Publication Date
    2014-04-08
    Print Publication Date
    2014-10
    Permanent link to this record
    http://hdl.handle.net/10754/563494
    
    Metadata
    Show full item record
    Abstract
    High-frequency (40 MHz) and low-frequency (7 MHz) capacitive micromachined ultrasound transducers (CMUT) were fabricated and tested for use in gravimetric detection of biomolecules. The low-frequency CMUT sensors have a gold-coated surface, while the high-frequency sensors have a silicon nitride surface. Both surfaces were functionalized with bovine leukemia virus antigen gp51 acting as the antigen. On addition of an a specific antibody labeled with horseradish peroxidase (HRP), the antigen/antibody complex is formed on the surface and quantified by HRP-catalyzed oxidation of tetramethylbenzidine. It has been found that a considerably smaller quantity of immuno complex is formed on the high frequency sensor surface. In parallel, the loading of the surface of the CMUT was determined via resonance frequency and electromechanical resistance readings. Following the formation of the immuno complexes, the resonance frequencies of the low-frequency and high-frequency sensors decrease by up to 420 and 440 kHz, respectively. Finite element analysis reveals that the loading of the (gold-coated) low frequency sensors is several times larger than that on high frequency sensors. The formation of the protein film with pronounced elasticity and stress on the gold surface case is discussed. We also discuss the adoption of this method for the detection of DNA using a hybridization assay following polymerase chain reaction.
    Citation
    Virzonis, D., Vanagas, G., Ramanaviciene, A., Makaraviciute, A., Barauskas, D., Ramanavicius, A., … Kodzius, R. (2014). Resonant gravimetric immunosensing based on capacitive micromachined ultrasound transducers. Microchimica Acta, 181(13-14), 1749–1757. doi:10.1007/s00604-014-1244-3
    Sponsors
    This research is funded by a grant (No. MIP-059/2012) from the Research Council of Lithuania.
    Publisher
    Springer Nature
    Journal
    Microchimica Acta
    DOI
    10.1007/s00604-014-1244-3
    ae974a485f413a2113503eed53cd6c53
    10.1007/s00604-014-1244-3
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Environmental Science and Engineering Program; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division

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