The polyadenylation factor subunit CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30: A key factor of programmed cell death and a regulator of immunity in arabidopsis
De Bont, Linda
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Plant Science Program
Desert Agriculture Initiative
Chromatin and development Research Group
MetadataShow full item record
AbstractProgrammed cell death (PCD) is essential for several aspects of plant life, including development and stress responses. Indeed, incompatible plant-pathogen interactions are well known to induce the hypersensitive response, a localized cell death. Mutational analyses have identified several key PCD components, and we recently identified the mips1 mutant of Arabidopsis (Arabidopsis thaliana), which is deficient for the key enzyme catalyzing the limiting step of myoinositol synthesis. One of the most striking features of mips1 is the light-dependent formation of lesions on leaves due to salicylic acid (SA)-dependent PCD, revealing roles for myoinositol or inositol derivatives in the regulation of PCD. Here, we identified a regulator of plant PCD by screening for mutants that display transcriptomic profiles opposing that of the mips1 mutant. Our screen identified the oxt6 mutant, which has been described previously as being tolerant to oxidative stress. In the oxt6 mutant, a transfer DNA is inserted in the CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30 (CPSF30) gene, which encodes a polyadenylation factor subunit homolog. We show that CPSF30 is required for lesion formation in mips1 via SA-dependent signaling, that the prodeath function of CPSF30 is not mediated by changes in the glutathione status, and that CPSF30 activity is required for Pseudomonas syringae resistance. We also show that the oxt6 mutation suppresses cell death in other lesion-mimic mutants, including lesion-simulating disease1, mitogen-activated protein kinase4, constitutive expressor of pathogenesis-related genes5, and catalase2, suggesting that CPSF30 and, thus, the control of messenger RNA 3′ end processing, through the regulation of SA production, is a key component of plant immune responses. © 2014 American Society of Plant Biologists. All rights reserved.
SponsorsThis work was supported by the Centre National de la Recherche Scientifique, the Universite Paris-Sud, the Agence Nationale de la Recherche (grant no. MAPK-IPS ANR-2010-BLAN-1613-02), and the Labex Saclay Plant Sciences.
PubMed Central IDPMC4044851
- Involvement of Arabidopsis Hexokinase1 in Cell Death Mediated by Myo-Inositol Accumulation.
- Authors: Bruggeman Q, Prunier F, Mazubert C, de Bont L, Garmier M, Lugan R, Benhamed M, Bergounioux C, Raynaud C, Delarue M
- Issue date: 2015 Jun
- Genome-wide control of polyadenylation site choice by CPSF30 in Arabidopsis.
- Authors: Thomas PE, Wu X, Liu M, Gaffney B, Ji G, Li QQ, Hunt AG
- Issue date: 2012 Nov
- A polyadenylation factor subunit implicated in regulating oxidative signaling in Arabidopsis thaliana.
- Authors: Zhang J, Addepalli B, Yun KY, Hunt AG, Xu R, Rao S, Li QQ, Falcone DL
- Issue date: 2008 Jun 11
- CPSF30 at the Interface of Alternative Polyadenylation and Cellular Signaling in Plants.
- Authors: Chakrabarti M, Hunt AG
- Issue date: 2015 Jun 8
- Integration of developmental and environmental signals via a polyadenylation factor in Arabidopsis.
- Authors: Liu M, Xu R, Merrill C, Hong L, Von Lanken C, Hunt AG, Li QQ
- Issue date: 2014