Show simple item record

dc.contributor.authorKyriacou, Bianca
dc.contributor.authorMoore, Katie L.
dc.contributor.authorPaterson, David J.
dc.contributor.authorDe Jonge, Martin Daly
dc.contributor.authorHoward, Daryl Lloyd
dc.contributor.authorStangoulis, James Constantine R
dc.contributor.authorTester, Mark A.
dc.contributor.authorLombi, E.
dc.contributor.authorJohnson, Alexander A T
dc.date.accessioned2015-08-03T11:50:56Z
dc.date.available2015-08-03T11:50:56Z
dc.date.issued2014-03
dc.identifier.citationKyriacou, B., Moore, K. L., Paterson, D., de Jonge, M. D., Howard, D. L., Stangoulis, J., … Johnson, A. A. T. (2014). Localization of iron in rice grain using synchrotron X-ray fluorescence microscopy and high resolution secondary ion mass spectrometry. Journal of Cereal Science, 59(2), 173–180. doi:10.1016/j.jcs.2013.12.006
dc.identifier.issn07335210
dc.identifier.doi10.1016/j.jcs.2013.12.006
dc.identifier.urihttp://hdl.handle.net/10754/563417
dc.description.abstractCereal crops accumulate low levels of iron (Fe) of which only a small fraction (5-10%) is bioavailable in human diets. Extensive co-localization of Fe in outer grain tissues with phytic acid, a strong chelator of metal ions, results in the formation of insoluble complexes that cannot be digested by humans. Here we describe the use of synchrotron X-ray fluorescence microscopy (XFM) and high resolution secondary ion mass spectrometry (NanoSIMS) to map the distribution of Fe, zinc (Zn), phosphorus (P) and other elements in the aleurone and subaleurone layers of mature grain from wild-type and an Fe-enriched line of rice (Oryza sativa L.). The results obtained from both XFM and NanoSIMS indicated that most Fe was co-localized with P (indicative of phytic acid) in the aleurone layer but that a small amount of Fe, often present as "hotspots", extended further into the subaleurone and outer endosperm in a pattern that was not co-localized with P. We hypothesize that Fe in subaleurone and outer endosperm layers of rice grain could be bound to low molecular weight chelators such as nicotianamine and/or deoxymugineic acid. © 2014.
dc.publisherElsevier BV
dc.subjectBioavailability
dc.subjectBiofortification
dc.subjectIP6
dc.subjectPhytate
dc.titleLocalization of iron in rice grain using synchrotron X-ray fluorescence microscopy and high resolution secondary ion mass spectrometry
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentPlant Science
dc.contributor.departmentPlant Science Program
dc.contributor.departmentThe Salt Lab
dc.identifier.journalJournal of Cereal Science
dc.contributor.institutionSchool of Biological Sciences, Flinders University of South Australia, GPO Box 2100, SA 5001, Australia
dc.contributor.institutionDepartment of Materials, University of Oxford, Parks Road, Oxford OX1 3PH, United Kingdom
dc.contributor.institutionAustralian Synchrotron, X-ray Fluorescence Microscopy, 800 Blackburn Road, Clayton, VIC 3168, Australia
dc.contributor.institutionCentre for Environmental Risk Assessment and Remediation, University of South Australia, Building X, Mawson Lakes Campus, Mawson Lakes, SA 5095, Australia
dc.contributor.institutionAustralian Centre for Plant Functional Genomics, University of Adelaide, PMB1, Glen Osmond, SA 5064, Australia
dc.contributor.institutionSchool of Botany, University of Melbourne, Victoria 3010, Australia
kaust.personTester, Mark A.


This item appears in the following Collection(s)

Show simple item record