Show simple item record

dc.contributor.authorToccafondi, Chiara
dc.contributor.authorThorat, Sanjay B.
dc.contributor.authorLa Rocca, Rosanna
dc.contributor.authorScarpellini, Alice
dc.contributor.authorSalerno, Marco
dc.contributor.authorDante, Silvia
dc.contributor.authorDas, Gobind
dc.date.accessioned2015-08-03T11:47:47Z
dc.date.available2015-08-03T11:47:47Z
dc.date.issued2014-02-27
dc.identifier.issn09574530
dc.identifier.doi10.1007/s10856-014-5178-4
dc.identifier.urihttp://hdl.handle.net/10754/563406
dc.description.abstractWe have fabricated anodic porous alumina from thin films (100/500 nm) of aluminium deposited on technological substrates of silicon/glass, and investigated the feasibility of this material as a surface for the development of analytical biosensors aiming to assess the status of living cells. To this goal, porous alumina surfaces with fixed pitch and variable pore size were analyzed for various functionalities. Gold coated (about 25 nm) alumina revealed surface enhanced Raman scattering increasing with the decrease in wall thickness, with factor up to values of approximately 104 with respect to the flat gold surface. Bare porous alumina was employed for micro-patterning and observation via fluorescence images of dye molecules, which demonstrated the surface capability for a drug-loading device. NIH-3T3 fibroblast cells were cultured in vitro and examined after 2 days since seeding, and no significant (P > 0.05) differences in their proliferation were observed on porous and non-porous materials. The effect on cell cultures of pore size in the range of 50–130 nm—with pore pitch of about 250 nm—showed no significant differences in cell viability and similar levels in all cases as on a control substrate. Future work will address combination of all above capabilities into a single device.
dc.publisherSpringer Nature
dc.titleMultifunctional substrates of thin porous alumina for cell biosensors
dc.typeArticle
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Division
dc.identifier.journalJournal of Materials Science: Materials in Medicine
dc.contributor.institutionNanophysics, Istituto Italiano di Tecnologia, via Morego 30Genoa, Italy
dc.contributor.institutionNanostructures, Istituto Italiano di Tecnologia, via Morego 30Genoa, Italy
dc.contributor.institutionUniversita‘ di Genova, viale Causa 13Genoa, Italy
dc.contributor.institutionNanochemistry, Istituto Italiano di Tecnologia, via Morego 30Genoa, Italy
kaust.personDas, Gobind
dc.date.published-online2014-02-27
dc.date.published-print2014-10


This item appears in the following Collection(s)

Show simple item record