Show simple item record

dc.contributor.authorShokry, Ahmed M.
dc.contributor.authorAl-Karim, Saleh
dc.contributor.authorRamadan, Ahmed M Ali
dc.contributor.authorGadallah, Nour
dc.contributor.authorAl-Attas, Sanaa G.
dc.contributor.authorSabir, Jamal Sabir M
dc.contributor.authorHassan, Sabah Mohammed
dc.contributor.authorMadkour, Loutfy H.
dc.contributor.authorBressan, Ray Anthony
dc.contributor.authorMahfouz, Magdy M.
dc.contributor.authorBahieldin, Ahmed M.
dc.date.accessioned2015-08-03T11:47:06Z
dc.date.available2015-08-03T11:47:06Z
dc.date.issued2014-02
dc.identifier.issn16310691
dc.identifier.pmid24581802
dc.identifier.doi10.1016/j.crvi.2013.12.008
dc.identifier.urihttp://hdl.handle.net/10754/563379
dc.description.abstractThe wild plant species Calotropis procera (C. procera) has many potential applications and beneficial uses in medicine, industry and ornamental field. It also represents an excellent source of genes for drought and salt tolerance. Genes encoding proteins that contain the conserved universal stress protein (USP) domain are known to provide organisms like bacteria, archaea, fungi, protozoa and plants with the ability to respond to a plethora of environmental stresses. However, information on the possible occurrence of Usp in C. procera is not available. In this study, we uncovered and characterized a one-class A Usp-like (UspA-like, NCBI accession No. KC954274) gene in this medicinal plant from the de novo assembled genome contigs of the high-throughput sequencing dataset. A number of GenBank accessions for Usp sequences were blasted with the recovered de novo assembled contigs. Homology modelling of the deduced amino acids (NCBI accession No. AGT02387) was further carried out using Swiss-Model, accessible via the EXPASY. Superimposition of C. procera USPA-like full sequence model on Thermus thermophilus USP UniProt protein (PDB accession No. Q5SJV7) was constructed using RasMol and Deep-View programs. The functional domains of the novel USPA-like amino acids sequence were identified from the NCBI conserved domain database (CDD) that provide insights into sequence structure/function relationships, as well as domain models imported from a number of external source databases (Pfam, SMART, COG, PRK, TIGRFAM). © 2014 Académie des sciences.
dc.description.sponsorshipThis project was funded by the Deanship of Scientific Research (DSR), King Abdulaziz University, Jeddah, under Grant No. 1432-3-15/HiCi. The authors, therefore, acknowledge with thanks DSR technical and financial support.
dc.publisherElsevier BV
dc.subject3D Modeling
dc.subjectAbiotic stress
dc.subjectBLAST
dc.subjectGTP-EFTU
dc.titleDetection of a Usp-like gene in Calotropis procera plant from the de novo assembled genome contigs of the high-throughput sequencing dataset
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentPlant Science Program
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentBioscience Program
dc.identifier.journalComptes Rendus Biologies
dc.contributor.institutionDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia
dc.contributor.institutionAgricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, Egypt
dc.contributor.institutionGenetics and Cytology Department, Genetic Engineering and Biotechnology Division, National Research Center, Dokki, Egypt
dc.contributor.institutionDepartment of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
dc.contributor.institutionArid Lands Agricultural Research Institute, Ain Shams University, Cairo, Egypt
dc.contributor.institutionSchool of Agriculture, Purdue University, West Lafayette, IN, United States
kaust.personMahfouz, Magdy M.


This item appears in the following Collection(s)

Show simple item record