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dc.contributor.authorZhang, Daming
dc.contributor.authorYang, Guang
dc.contributor.authorChen, Xin
dc.contributor.authorLi, Chunmei
dc.contributor.authorWang, Lu
dc.contributor.authorLiu, Yaohua
dc.contributor.authorHan, Dayong
dc.contributor.authorLiu, Huailei
dc.contributor.authorHou, Xu
dc.contributor.authorZhang, Weiguang
dc.contributor.authorLi, Chenguang
dc.contributor.authorHan, Zhanqiang
dc.contributor.authorGao, Xin
dc.contributor.authorZhao, Shiguang
dc.date.accessioned2015-08-03T11:46:37Z
dc.date.available2015-08-03T11:46:37Z
dc.date.issued2014-01-28
dc.identifier.issn08958696
dc.identifier.doi10.1007/s12031-014-0230-x
dc.identifier.urihttp://hdl.handle.net/10754/563358
dc.description.abstractMicroRNAs (miRNAs) are small noncoding RNAs that have been critically implicated in several human cancers. miRNAs are thought to participate in various biological processes, including proliferation, cell cycle, apoptosis, and even the regulation of the stemness properties of cancer stem cells. In this study, we explore the potential role of miR-300 in glioma stem-like cells (GSLCs). We isolated GSLCs from glioma biopsy specimens and identified the stemness properties of the cells through neurosphere formation assays, multilineage differentiation ability analysis, and immunofluorescence analysis of glioma stem cell markers. We found that miR-300 is commonly upregulated in glioma tissues, and the expression of miR-300 was higher in GSLCs. The results of functional experiments demonstrated that miR-300 can enhance the self-renewal of GSLCs and reduce differentiation toward both astrocyte and neural fates. In addition, LZTS2 is a direct target of miR-300. In conclusion, our results demonstrate the critical role of miR-300 in GSLCs and its functions in LZTS2 inhibition and describe a new approach for the molecular regulation of tumor stem cells. © 2014 Springer Science+Business Media.
dc.description.sponsorshipThis work was supported by the National Natural Science Foundation of China (grant numbers 81272788 and 81302178), the Natural Science Foundation of Heilongjiang (QC2013C096), and the Fund of the First Affiliated Hospital of Harbin Medical University (2013B01).
dc.publisherSpringer Nature
dc.subjectDifferentiation
dc.subjectGlioma stem-like cells
dc.subjectLZTS2
dc.subjectmiR-300
dc.subjectSelf-renewal
dc.titlemir-300 promotes self-renewal and inhibits the differentiation of glioma stem-like cells
dc.typeArticle
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentComputer Science Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentStructural and Functional Bioinformatics Group
dc.identifier.journalJournal of Molecular Neuroscience
dc.contributor.institutionDepartment of Neurosurgery, Harbin Medical University, Youzheng Street No. 23, Nangang District, Harbin, Heilongjiang Province 150001, China
dc.contributor.institutionInstitute of Brain Science, Harbin Medical University, Harbin, Heilongjiang, China
dc.contributor.institutionDepartment of Neurology, Harbin Medical University, Harbin, Heilongjiang, China
dc.contributor.institutionDepartment of Pharmacology (State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), Harbin Medical University, Harbin, Heilongjiang, China
kaust.personGao, Xin
dc.date.published-online2014-01-28
dc.date.published-print2014-08


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