mir-300 promotes self-renewal and inhibits the differentiation of glioma stem-like cells
Type
ArticleAuthors
Zhang, DamingYang, Guang
Chen, Xin
Li, Chunmei
Wang, Lu
Liu, Yaohua
Han, Dayong
Liu, Huailei
Hou, Xu
Zhang, Weiguang
Li, Chenguang
Han, Zhanqiang
Gao, Xin

Zhao, Shiguang
KAUST Department
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) DivisionComputer Science Program
Computational Bioscience Research Center (CBRC)
Structural and Functional Bioinformatics Group
Date
2014-01-28Online Publication Date
2014-01-28Print Publication Date
2014-08Permanent link to this record
http://hdl.handle.net/10754/563358
Metadata
Show full item recordAbstract
MicroRNAs (miRNAs) are small noncoding RNAs that have been critically implicated in several human cancers. miRNAs are thought to participate in various biological processes, including proliferation, cell cycle, apoptosis, and even the regulation of the stemness properties of cancer stem cells. In this study, we explore the potential role of miR-300 in glioma stem-like cells (GSLCs). We isolated GSLCs from glioma biopsy specimens and identified the stemness properties of the cells through neurosphere formation assays, multilineage differentiation ability analysis, and immunofluorescence analysis of glioma stem cell markers. We found that miR-300 is commonly upregulated in glioma tissues, and the expression of miR-300 was higher in GSLCs. The results of functional experiments demonstrated that miR-300 can enhance the self-renewal of GSLCs and reduce differentiation toward both astrocyte and neural fates. In addition, LZTS2 is a direct target of miR-300. In conclusion, our results demonstrate the critical role of miR-300 in GSLCs and its functions in LZTS2 inhibition and describe a new approach for the molecular regulation of tumor stem cells. © 2014 Springer Science+Business Media.Citation
Zhang, D., Yang, G., Chen, X., Li, C., Wang, L., Liu, Y., … Zhao, S. (2014). mir-300 Promotes Self-Renewal and Inhibits the Differentiation of Glioma Stem-Like Cells. Journal of Molecular Neuroscience, 53(4), 637–644. doi:10.1007/s12031-014-0230-xSponsors
This work was supported by the National Natural Science Foundation of China (grant numbers 81272788 and 81302178), the Natural Science Foundation of Heilongjiang (QC2013C096), and the Fund of the First Affiliated Hospital of Harbin Medical University (2013B01).Publisher
Springer Natureae974a485f413a2113503eed53cd6c53
10.1007/s12031-014-0230-x