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    mir-300 promotes self-renewal and inhibits the differentiation of glioma stem-like cells

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    Type
    Article
    Authors
    Zhang, Daming
    Yang, Guang
    Chen, Xin
    Li, Chunmei
    Wang, Lu
    Liu, Yaohua
    Han, Dayong
    Liu, Huailei
    Hou, Xu
    Zhang, Weiguang
    Li, Chenguang
    Han, Zhanqiang
    Gao, Xin cc
    Zhao, Shiguang
    KAUST Department
    Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
    Computer Science Program
    Computational Bioscience Research Center (CBRC)
    Structural and Functional Bioinformatics Group
    Date
    2014-01-28
    Online Publication Date
    2014-01-28
    Print Publication Date
    2014-08
    Permanent link to this record
    http://hdl.handle.net/10754/563358
    
    Metadata
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    Abstract
    MicroRNAs (miRNAs) are small noncoding RNAs that have been critically implicated in several human cancers. miRNAs are thought to participate in various biological processes, including proliferation, cell cycle, apoptosis, and even the regulation of the stemness properties of cancer stem cells. In this study, we explore the potential role of miR-300 in glioma stem-like cells (GSLCs). We isolated GSLCs from glioma biopsy specimens and identified the stemness properties of the cells through neurosphere formation assays, multilineage differentiation ability analysis, and immunofluorescence analysis of glioma stem cell markers. We found that miR-300 is commonly upregulated in glioma tissues, and the expression of miR-300 was higher in GSLCs. The results of functional experiments demonstrated that miR-300 can enhance the self-renewal of GSLCs and reduce differentiation toward both astrocyte and neural fates. In addition, LZTS2 is a direct target of miR-300. In conclusion, our results demonstrate the critical role of miR-300 in GSLCs and its functions in LZTS2 inhibition and describe a new approach for the molecular regulation of tumor stem cells. © 2014 Springer Science+Business Media.
    Citation
    Zhang, D., Yang, G., Chen, X., Li, C., Wang, L., Liu, Y., … Zhao, S. (2014). mir-300 Promotes Self-Renewal and Inhibits the Differentiation of Glioma Stem-Like Cells. Journal of Molecular Neuroscience, 53(4), 637–644. doi:10.1007/s12031-014-0230-x
    Sponsors
    This work was supported by the National Natural Science Foundation of China (grant numbers 81272788 and 81302178), the Natural Science Foundation of Heilongjiang (QC2013C096), and the Fund of the First Affiliated Hospital of Harbin Medical University (2013B01).
    Publisher
    Springer Nature
    Journal
    Journal of Molecular Neuroscience
    DOI
    10.1007/s12031-014-0230-x
    ae974a485f413a2113503eed53cd6c53
    10.1007/s12031-014-0230-x
    Scopus Count
    Collections
    Articles; Structural and Functional Bioinformatics Group; Computer Science Program; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division

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