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    Identification of redox-sensitive cysteines in the arabidopsis proteome using OxiTRAQ, a quantitative redox proteomics method

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    Type
    Article
    Authors
    Liu, Pei-Nian cc
    Zhang, Huoming cc
    Wang, Hai
    Xia, Yiji
    KAUST Department
    Bioscience Core Lab
    Date
    2014-01-28
    Online Publication Date
    2014-01-28
    Print Publication Date
    2014-03
    Permanent link to this record
    http://hdl.handle.net/10754/563356
    
    Metadata
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    Abstract
    Cellular redox status plays a key role in mediating various physiological and developmental processes often through modulating activities of redox-sensitive proteins. Various stresses trigger over-production of reactive oxygen/nitrogen species which lead to oxidative modifications of redox-sensitive proteins. Identification and characterization of redox-sensitive proteins are important steps toward understanding molecular mechanisms of stress responses. Here, we report a high-throughput quantitative proteomic approach termed OxiTRAQ for identifying proteins whose thiols undergo reversible oxidative modifications in Arabidopsis cells subjected to oxidative stress. In this approach, a biotinylated thiol-reactive reagent is used for differential labeling of reduced and oxidized thiols. The biotin-tagged peptides are affinity purified, labeled with iTRAQ reagents, and analyzed using a paralleled HCD-CID fragmentation mode in an LTQ-Orbitrap. With this approach, we identified 195 cysteine-containing peptides from 179 proteins whose thiols underwent oxidative modifications in Arabidopsis cells following the treatment with hydrogen peroxide. A majority of those redox-sensitive proteins, including several transcription factors, were not identified by previous redox proteomics studies. This approach allows identification of the specific redox-regulated cysteine residues, and offers an effective tool for elucidation of redox proteomes. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
    Sponsors
    This work was supported by Research Grants Council of Hong Kong (grant nos. HKBU1/CRF/10 and HKBU261910 to Y.X.) and by HKBU Strategic Development Fund (to Y.X.).
    Publisher
    Wiley
    Journal
    PROTEOMICS
    DOI
    10.1002/pmic.201300307
    PubMed ID
    24376095
    ae974a485f413a2113503eed53cd6c53
    10.1002/pmic.201300307
    Scopus Count
    Collections
    Articles; Bioscience Core Lab

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