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The chloroplast genome.pdf
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Accepted manuscript
Type
ArticleKAUST Department
Red Sea Research Center (RSRC)Biological and Environmental Sciences and Engineering (BESE) Division
Marine Science Program
Reef Genomics Lab
Date
2013-10-17Embargo End Date
2014-10-17Permanent link to this record
http://hdl.handle.net/10754/563301
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Dinoflagellate algae of the genus Symbiodinium form important symbioses within corals and other benthic marine animals. Dinoflagellates possess an extremely reduced plastid genome relative to those examined in plants and other algae. In dinoflagellates the plastid genes are located on small plasmids, commonly referred to as 'minicircles'. However, the chloroplast genomes of dinoflagellates have only been extensively characterised from a handful of species. There is also evidence of considerable variation in the chloroplast genome organisation across those species that have been examined. We therefore characterised the chloroplast genome from an environmental coral isolate, in this case containing a symbiont belonging to the Symbiodinium sp. clade C3. The gene content of the genome is well conserved with respect to previously characterised genomes. However, unlike previously characterised dinoflagellate chloroplast genomes we did not identify any 'empty' minicircles. The sequences of this chloroplast genome show a high rate of evolution relative to other algal species. Particularly notable was a surprisingly high level of sequence divergence within the core polypeptides of photosystem I, the reasons for which are currently unknown. This chloroplast genome also possesses distinctive codon usage and GC content. These features suggest that chloroplast genomes in Symbiodinium are highly plastic. © 2013 Adrian C. Barbrook.Citation
Barbrook, A. C., Voolstra, C. R., & Howe, C. J. (2014). The Chloroplast Genome of a Symbiodinium sp. Clade C3 Isolate. Protist, 165(1), 1–13. doi:10.1016/j.protis.2013.09.006Sponsors
The authors wish to thank Professor Angela Douglas for the gift of the DNA extract used in this study. The authors also wish to thank Daniel J. Thornhilland Todd J. LaJeunesse for providing assistance inphylogenetic assignment of the Symbiodinium isolate. This work was funded through a grant from the Cambridge - KAUST Academic Excellence Alliance (AEA) program (award number 7000000056) as well as by the Leverhulme Trust.Publisher
Elsevier BVJournal
ProtistPubMed ID
24316380Additional Links
https://doi.org/10.1016/j.protis.2013.09.006ae974a485f413a2113503eed53cd6c53
10.1016/j.protis.2013.09.006
Scopus Count
Except where otherwise noted, this item's license is described as This is an open access article made available under the CC-BY-NC-ND 4.0 license.
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