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    High throughput second harmonic imaging for label-free biological applications

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    Type
    Article
    Authors
    Macias Romero, Carlos
    Didier, Marie E P
    Jourdain, Pascal
    Marquet, Pierre
    Magistretti, Pierre J. cc
    Tarun, Orly B.
    Zubkovs, Vitalijs
    Radenovic, Aleksandra
    Roke, Sylvie
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Date
    2014-12-08
    Online Publication Date
    2014-12-08
    Print Publication Date
    2014-12-15
    Permanent link to this record
    http://hdl.handle.net/10754/563269
    
    Metadata
    Show full item record
    Abstract
    Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging throughput of SHG microscopy by using a wide-field imaging scheme in combination with a medium-range repetition rate amplified near infrared femtosecond laser source and gated detection. The imaging throughput of this configuration is tested by measuring the optical image contrast for different image acquisition times of BaTiO3 nanoparticles in two different wide-field setups and one commercial point-scanning configuration. We find that the second harmonic imaging throughput is improved by 2-3 orders of magnitude compared to point-scan imaging. Capitalizing on this result, we perform low fluence imaging of (parts of) living mammalian neurons in culture.
    Sponsors
    This work was supported by the Julia Jacobi Foundation, the Swiss National Foundation (grant number 200021-146884), and European Commission, Research Executive Agency Marie Curie Actions 'FINON' (ITN-2013-607842). We would like to thank Prof. K. B. Eisenthal for very inspiring discussions.
    Publisher
    The Optical Society
    Journal
    Optics Express
    DOI
    10.1364/OE.22.031102
    ae974a485f413a2113503eed53cd6c53
    10.1364/OE.22.031102
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program

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