High throughput second harmonic imaging for label-free biological applications
Type
ArticleAuthors
Macias Romero, CarlosDidier, Marie E P
Jourdain, Pascal
Marquet, Pierre
Magistretti, Pierre J.

Tarun, Orly B.
Zubkovs, Vitalijs
Radenovic, Aleksandra
Roke, Sylvie
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Date
2014-12-08Online Publication Date
2014-12-08Print Publication Date
2014-12-15Permanent link to this record
http://hdl.handle.net/10754/563269
Metadata
Show full item recordAbstract
Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging throughput of SHG microscopy by using a wide-field imaging scheme in combination with a medium-range repetition rate amplified near infrared femtosecond laser source and gated detection. The imaging throughput of this configuration is tested by measuring the optical image contrast for different image acquisition times of BaTiO3 nanoparticles in two different wide-field setups and one commercial point-scanning configuration. We find that the second harmonic imaging throughput is improved by 2-3 orders of magnitude compared to point-scan imaging. Capitalizing on this result, we perform low fluence imaging of (parts of) living mammalian neurons in culture.Sponsors
This work was supported by the Julia Jacobi Foundation, the Swiss National Foundation (grant number 200021-146884), and European Commission, Research Executive Agency Marie Curie Actions 'FINON' (ITN-2013-607842). We would like to thank Prof. K. B. Eisenthal for very inspiring discussions.Publisher
The Optical SocietyJournal
Optics Expressae974a485f413a2113503eed53cd6c53
10.1364/OE.22.031102