High throughput second harmonic imaging for label-free biological applications
AuthorsMacias Romero, Carlos
Didier, Marie E P
Magistretti, Pierre J.
Tarun, Orly B.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
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AbstractSecond harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging throughput of SHG microscopy by using a wide-field imaging scheme in combination with a medium-range repetition rate amplified near infrared femtosecond laser source and gated detection. The imaging throughput of this configuration is tested by measuring the optical image contrast for different image acquisition times of BaTiO3 nanoparticles in two different wide-field setups and one commercial point-scanning configuration. We find that the second harmonic imaging throughput is improved by 2-3 orders of magnitude compared to point-scan imaging. Capitalizing on this result, we perform low fluence imaging of (parts of) living mammalian neurons in culture.
SponsorsThis work was supported by the Julia Jacobi Foundation, the Swiss National Foundation (grant number 200021-146884), and European Commission, Research Executive Agency Marie Curie Actions 'FINON' (ITN-2013-607842). We would like to thank Prof. K. B. Eisenthal for very inspiring discussions.
PublisherThe Optical Society