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    Galectin-3 coats the membrane of breast cells and makes a signature of tumours

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    Type
    Article
    Authors
    Simone, Giuseppina
    Malara, Natalia Maria
    Trunzo, Valentina
    Renne, Maria
    Perozziello, Gerardo
    Di Fabrizio, Enzo M. cc
    Manz, Andreas
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Physical Sciences and Engineering (PSE) Division
    Materials Science and Engineering Program
    Date
    2014
    Permanent link to this record
    http://hdl.handle.net/10754/563168
    
    Metadata
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    Abstract
    Galectin-3, β-galactoside-binding lectin, coats the membrane of most cancer cells and is involved in metastasis and endothelium recognition as well as in evading immune surveillance through killing of activated T cells. To flag galectin as a biomarker of tumours and metastasis, it is pivotal to understand the role of this protein in different tumours and at different stages. Breast tumours have an anomalous behaviour of the galectin-3 compared to other tumour cells. Herein, FACS sorting and galactoside based assays were used to investigate the role of galectin-3 in metastasis and metastatisation of breast cancer cells. Breast galectin fingerprint at the FACS displayed a higher amount in healthy cells, compared to metastatic cells. The microfluidic assay was able to isolate tumour and metastatic cells more than healthy breast cells. Investigation was performed on samples from patients with breast tumours at stage I and stage III whilst MCF7 and EPH-4 cells were used to perform preliminary investigations. The readout of the conditioned medium (from culturing of stage I cells) fingerprint by FACS evidenced high expression of free galectin. Analysis of the results established that the galectin coating the membrane, by galactoside recognition of the breast cells, and engaged by the cells to form protein-carbohydrate complexes inside the microfluidic assay, resembled the tumour signature of tumours in breast cells whilst the galectin free is independent of those mechanisms. © 2014 The Royal Society of Chemistry.
    Sponsors
    This work was partially supported by the project PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti.'' (cod. PON01_02782, CUP B71H11000870005), and the project FIRB "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet'' (cod. RBPR05JH2P_010, CUP B41J09000110005) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro, the foundation "T. Campanella'' and RSA (ANASTE Calabria). The authors are grateful to Prof. Torella, Prof. Agosti, Prof. Fresta. Moreover, the authors are grateful to all patients and relatives involved in this study.
    Publisher
    Royal Society of Chemistry (RSC)
    Journal
    Mol. BioSyst.
    DOI
    10.1039/c3mb70359b
    PubMed ID
    24281352
    ae974a485f413a2113503eed53cd6c53
    10.1039/c3mb70359b
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program

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