Tissue- and stage-dependent dosage compensation on the Neo-X chromosome in drosophila pseudoobscura
KAUST DepartmentComputational Bioscience Research Center (CBRC)
Biological and Environmental Sciences and Engineering (BESE) Division
Online Publication Date2013-12-03
Print Publication Date2014-03
Permanent link to this recordhttp://hdl.handle.net/10754/563139
MetadataShow full item record
AbstractSex chromosome dosage compensation (DC) is widely accepted in various organisms. This concept is mostly supported by comparisons of gene expression between chromosomes and between sexes. However, genes on the X chromosome and autosomes are mostly not homologous, and the average gene expression level on these chromosomes may not be the same even under DC, which complicates comparisons between chromosomes. Many genes with sex-biased expression also make comparisons between sexes difficult. To overcome these issues, we investigated DC by comparing the expression of neo-X-linked genes in Drosophila pseudoobscura with those of their autosomal orthologs in other Drosophila species. The ratio of the former to the latter in males would be 1 under DC, whereas it becomes 0.5 without DC. We found that the ratio was ∼0.85 for adult whole bodies, indicating that the DC is incomplete on the neo-X chromosome in adults as a whole. The ratio (∼0.90) was also significantly less than 1 for adult bodies without gonads, whereas it was ∼1.0 for adult heads. These results indicate that DC varies among tissues. Our sliding-window analysis of the ratio also revealed that the upregulation of neo-X-linked genes in males occurred chromosome wide in all tissues analyzed, indicating global upregulation mechanisms. However, we found that gene functions also affected the levels of DC. Furthermore, most of the genes recently moved to the X were already under DC at the larval stage but not at the adult stage. These results suggest that DC in Drosophila species operates in a tissue/stage-dependent manner. © 2013 The Author 2013. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved.
CitationNozawa, M., Fukuda, N., Ikeo, K., & Gojobori, T. (2013). Tissue- and Stage-Dependent Dosage Compensation on the Neo-X Chromosome in Drosophila pseudoobscura. Molecular Biology and Evolution, 31(3), 614–624. doi:10.1093/molbev/mst239
PublisherOxford University Press (OUP)
JournalMolecular Biology and Evolution
- Gene-by-Gene or Localized Dosage Compensation on the Neo-X Chromosome in Drosophila miranda.
- Authors: Nozawa M, Ikeo K, Gojobori T
- Issue date: 2018 Aug 1
- Evolution of sex chromosomes: dosage compensation of the Lcp1-4 gene cluster on the evolving neo-X chromosome in Drosophila miranda.
- Authors: Steinemann S, Steinemann M
- Issue date: 2007 Apr
- The dosage compensation system of Drosophila is co-opted by newly evolved X chromosomes.
- Authors: Marín I, Franke A, Bashaw GJ, Baker BS
- Issue date: 1996 Sep 12
- Complete Dosage Compensation in Anopheles stephensi and the Evolution of Sex-Biased Genes in Mosquitoes.
- Authors: Jiang X, Biedler JK, Qi Y, Hall AB, Tu Z
- Issue date: 2015 Jun 15
- Parallel Universes for Models of X Chromosome Dosage Compensation in Drosophila: A Review.
- Authors: Birchler JA
- Issue date: 2016