Batf3 and Id2 have a synergistic effect on Irf8-directed classical CD8α+ dendritic cell development
Type
ArticleAuthors
Jaiswal, HemantKaushik, Monika
Sougrat, Rachid

Gupta, Monica
Dey, Anup
Verma, Rohit
Ozato, Keiko
Tailor, Prafullakumar B.
KAUST Department
Imaging and Characterization Core LabAdvanced Nanofabrication, Imaging and Characterization Core Lab
Core Labs
Date
2013-11-13Online Publication Date
2013-11-13Print Publication Date
2013-12-15Permanent link to this record
http://hdl.handle.net/10754/563084
Metadata
Show full item recordAbstract
Dendritic cells (DCs) are heterogeneous cell populations represented by different subtypes, each varying in terms of gene expression patterns and specific functions. Recent studies identified transcription factors essential for the development of different DC subtypes, yet molecular mechanisms for the developmental program and functions remain poorly understood. In this study, we developed and characterized a mouse DC progenitor-like cell line, designated DC9, from Irf8-/- bone marrow cells as a model for DC development and function. Expression of Irf8 in DC9 cells led to plasmacytoid DCs and CD8α+ DC-like cells, with a concomitant increase in plasmacytoid DC- and CD8α+ DC-specific gene transcripts and induction of type I IFNs and IL12p40 following TLR ligand stimulation. Irf8 expression in DC9 cells led to an increase in Id2 and Batf3 transcript levels, transcription factors shown to be important for the development of CD8α+ DCs. We show that, without Irf8 , expression of Id2 and Batf3 was not sufficient for directing classical CD8α+ DC development. When coexpressed with Irf8, Batf3 and Id2 had a synergistic effect on classical CD8α+ DC development. We demonstrate that Irf8 is upstream of Batf3 and Id2 in the classical CD8α+ DC developmental program and define the hierarchical relationship of transcription factors important for classical CD8α+ DC development.Citation
Jaiswal, H., Kaushik, M., Sougrat, R., Gupta, M., Dey, A., Verma, R., … Tailor, P. (2013). Batf3 and Id2 Have a Synergistic Effect on Irf8-Directed Classical CD8α+ Dendritic Cell Development. The Journal of Immunology, 191(12), 5993–6001. doi:10.4049/jimmunol.1203541Sponsors
This work was supported by the National Institute of Immunology Core Fund. P.T. is a Ramalingaswami fellow, Department of Biotechnology, "Government of India" at National Institute of Immunology. H.J. and R.V. are supported by a fellowship from the Council for Scientific and Industrial Research, Government of India. M.K. is supported by a contingency grant of the Ramalingaswami Fellowship awarded to P.T.Journal
The Journal of ImmunologyPubMed ID
24227775ae974a485f413a2113503eed53cd6c53
10.4049/jimmunol.1203541
Scopus Count
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