• Login
    View Item 
    •   Home
    • Research
    • Articles
    • View Item
    •   Home
    • Research
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguideTheses and Dissertations LibguideSubmit an Item

    Statistics

    Display statistics

    Batf3 and Id2 have a synergistic effect on Irf8-directed classical CD8α+ dendritic cell development

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Type
    Article
    Authors
    Jaiswal, Hemant
    Kaushik, Monika
    Sougrat, Rachid cc
    Gupta, Monica
    Dey, Anup
    Verma, Rohit
    Ozato, Keiko
    Tailor, Prafullakumar B.
    KAUST Department
    Imaging and Characterization Core Lab
    Advanced Nanofabrication, Imaging and Characterization Core Lab
    Core Labs
    Date
    2013-11-13
    Online Publication Date
    2013-11-13
    Print Publication Date
    2013-12-15
    Permanent link to this record
    http://hdl.handle.net/10754/563084
    
    Metadata
    Show full item record
    Abstract
    Dendritic cells (DCs) are heterogeneous cell populations represented by different subtypes, each varying in terms of gene expression patterns and specific functions. Recent studies identified transcription factors essential for the development of different DC subtypes, yet molecular mechanisms for the developmental program and functions remain poorly understood. In this study, we developed and characterized a mouse DC progenitor-like cell line, designated DC9, from Irf8-/- bone marrow cells as a model for DC development and function. Expression of Irf8 in DC9 cells led to plasmacytoid DCs and CD8α+ DC-like cells, with a concomitant increase in plasmacytoid DC- and CD8α+ DC-specific gene transcripts and induction of type I IFNs and IL12p40 following TLR ligand stimulation. Irf8 expression in DC9 cells led to an increase in Id2 and Batf3 transcript levels, transcription factors shown to be important for the development of CD8α+ DCs. We show that, without Irf8 , expression of Id2 and Batf3 was not sufficient for directing classical CD8α+ DC development. When coexpressed with Irf8, Batf3 and Id2 had a synergistic effect on classical CD8α+ DC development. We demonstrate that Irf8 is upstream of Batf3 and Id2 in the classical CD8α+ DC developmental program and define the hierarchical relationship of transcription factors important for classical CD8α+ DC development.
    Citation
    Jaiswal, H., Kaushik, M., Sougrat, R., Gupta, M., Dey, A., Verma, R., … Tailor, P. (2013). Batf3 and Id2 Have a Synergistic Effect on Irf8-Directed Classical CD8α+ Dendritic Cell Development. The Journal of Immunology, 191(12), 5993–6001. doi:10.4049/jimmunol.1203541
    Sponsors
    This work was supported by the National Institute of Immunology Core Fund. P.T. is a Ramalingaswami fellow, Department of Biotechnology, "Government of India" at National Institute of Immunology. H.J. and R.V. are supported by a fellowship from the Council for Scientific and Industrial Research, Government of India. M.K. is supported by a contingency grant of the Ramalingaswami Fellowship awarded to P.T.
    Publisher
    The American Association of Immunologists
    Journal
    The Journal of Immunology
    DOI
    10.4049/jimmunol.1203541
    PubMed ID
    24227775
    ae974a485f413a2113503eed53cd6c53
    10.4049/jimmunol.1203541
    Scopus Count
    Collections
    Articles; Imaging and Characterization Core Lab

    entitlement

    Related articles

    • CD8α+ DCs can be induced in the absence of transcription factors Id2, Nfil3, and Batf3.
    • Authors: Seillet C, Jackson JT, Markey KA, Brady HJ, Hill GR, Macdonald KP, Nutt SL, Belz GT
    • Issue date: 2013 Feb 28
    • Batf3 maintains autoactivation of Irf8 for commitment of a CD8α(+) conventional DC clonogenic progenitor.
    • Authors: Grajales-Reyes GE, Iwata A, Albring J, Wu X, Tussiwand R, Kc W, Kretzer NM, Briseño CG, Durai V, Bagadia P, Haldar M, Schönheit J, Rosenbauer F, Murphy TL, Murphy KM
    • Issue date: 2015 Jul
    • IRF8 and BATF3 interaction enhances the cDC1 specific Pfkfb3 gene expression.
    • Authors: Chauhan KS, Das A, Jaiswal H, Saha I, Kaushik M, Patel VK, Tailor P
    • Issue date: 2022 Jan
    • Peripheral CD103+ dendritic cells form a unified subset developmentally related to CD8alpha+ conventional dendritic cells.
    • Authors: Edelson BT, KC W, Juang R, Kohyama M, Benoit LA, Klekotka PA, Moon C, Albring JC, Ise W, Michael DG, Bhattacharya D, Stappenbeck TS, Holtzman MJ, Sung SS, Murphy TL, Hildner K, Murphy KM
    • Issue date: 2010 Apr 12
    • NFIL3/E4BP4 is a key transcription factor for CD8α⁺ dendritic cell development.
    • Authors: Kashiwada M, Pham NL, Pewe LL, Harty JT, Rothman PB
    • Issue date: 2011 Jun 9
    DSpace software copyright © 2002-2023  DuraSpace
    Quick Guide | Contact Us | KAUST University Library
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.