Understanding the regulation of estivation in a freshwater snail through iTRAQ-based comparative proteomics
Chandramouli, Kondethimmanahalli H.
KAUST DepartmentBioscience Core Lab
Online Publication Date2013-10-02
Print Publication Date2013-11
Permanent link to this recordhttp://hdl.handle.net/10754/563073
MetadataShow full item record
AbstractThe apple snail Pomacea canaliculata is a freshwater gastropod with a remarkable ability to withstand seasonal or unpredictable dry conditions by entering estivation. Studies of P. canaliculata using conventional biochemical and the individual gene approaches have revealed the expressional changes of several enzymes and antioxidative genes in response to estivation and arousal. In this study, we applied iTRAQ-coupled two-dimensional LC-MS/MS to identify and quantify the global protein expression during the estivation and arousal of P. canaliculata. A total of 1040 proteins were identified, among which 701 proteins were quantified and compared across four treatments (i.e., control, active snails; short-term estivation, 3 days of exposure to air; prolonged estivation, 30 days of exposure to air; and arousal, 6 h after resubmergence in water) revealing 53 differentially expressed proteins. A comparison of protein expression profiles across treatments indicated that the proteome of this species was very insensitive to initial estivation, with only 9 proteins differentially expressed as compared with the control. Among the 9 proteins, the up-regulations of two immune related proteins indicated the initial immune response to the detection of stress cues. Prolonged estivation resulted in many more differentially expressed proteins (47 compared with short-term estivation treatment), among which 16 were down-regulated and 31 were up-regulated. These differentially expressed proteins have provided the first global picture of a shift in energy usage from glucose to lipid, prevention of protein degradation and elevation of oxidative defense, and production of purine for uric acid production to remove toxic ammonia during prolonged estivation in a freshwater snail. From prolonged estivation to arousal, only 6 proteins changed their expression level, indicating that access to water and food alone is not a necessary condition to reactivate whole-sale protein expression. A comparison with hibernation and diapause revealed many similar molecular mechanisms of hypometabolic regulation across the animal kingdom. © 2013 American Chemical Society.
CitationSun, J., Mu, H., Zhang, H., Chandramouli, K. H., Qian, P.-Y., Wong, C. K. C., & Qiu, J.-W. (2013). Understanding the Regulation of Estivation in a Freshwater Snail through iTRAQ-Based Comparative Proteomics. Journal of Proteome Research, 12(11), 5271–5280. doi:10.1021/pr400570a
SponsorsWe thank Prof. Horacio Heras for his helpful comments on an early draft of the manuscript, Prof. Alfredo Castro-Vazquez for clarifying the use of anatomical terminology in apple snails, and anonymous reviewers for their critical comments on the manuscript. This study was supported by a grant (GRF 261312) from the University Grant Committee.
PublisherAmerican Chemical Society (ACS)
JournalJournal of Proteome Research
CollectionsArticles; Bioscience Core Lab
- Genetic Basis of Differential Heat Resistance between Two Species of Congeneric Freshwater Snails: Insights from Quantitative Proteomics and Base Substitution Rate Analysis.
- Authors: Mu H, Sun J, Fang L, Luan T, Williams GA, Cheung SG, Wong CK, Qiu JW
- Issue date: 2015 Oct 2
- Uric acid deposits and estivation in the invasive apple-snail, Pomacea canaliculata.
- Authors: Giraud-Billoud M, Abud MA, Cueto JA, Vega IA, Castro-Vazquez A
- Issue date: 2011 Apr
- Antioxidant and molecular chaperone defences during estivation and arousal in the South American apple snail Pomacea canaliculata.
- Authors: Giraud-Billoud M, Vega IA, Tosi ME, Abud MA, Calderón ML, Castro-Vazquez A
- Issue date: 2013 Feb 15
- Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study.
- Authors: Chen M, Li X, Zhu A, Storey KB, Sun L, Gao T, Wang T
- Issue date: 2016 Sep
- Rapid upregulation of heart antioxidant enzymes during arousal from estivation in the Giant African snail (Achatina fulica).
- Authors: Salway KD, Tattersall GJ, Stuart JA
- Issue date: 2010 Nov