A microfluidic device integrating plasmonic nanodevices for Raman spectroscopy analysis on trapped single living cells
De Angelis, Francesco De
Malara, Natalia Maria
Di Fabrizio, Enzo M.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Physical Sciences and Engineering (PSE) Division
Materials Science and Engineering Program
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AbstractIn this work we developed a microfluidic device integrating nanoplasmonic devices combined with fluidic trapping regions. The microfuidic traps allow to capture single cells in areas where plasmonic sensors are placed. In this way it is possible to perform Enhanced Raman analysis on the cell membranes. Moreover, by changing direction of the flux it is possible to change the orientation of the cell in the trap, so that it is possible to analyze different points of the membrane of the same cell. We shows an innovative procedure to fabricate and assembly the microfluidic device which combine photolithography, focused ion beam machining, and hybrid bonding between a polymer substrate and lid of Calcium fluoride. This procedure is compatible with the fabrication of the plasmonic sensors in close proximity of the microfluidic traps. Moreover, the use of Calcium fluoride as lid allows full compatibility with Raman measurements producing negligible Raman background signal and avoids Raman artifacts. Finally, we performed Raman analysis on cells to monitor their oxidative stress under particular non physiological conditions. © 2013 Elsevier B.V. All rights reserved.
SponsorsThis work was partially supported by the project PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti" (cod.PON01_02782, CUP B71H11000870005), the project FIRB "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" (cod.RBPR05JH2P_010, CUP B41 J09000110005) and Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant No. 2011-0338) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University "Magna Graecia" of Catanzaro.