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dc.contributor.authorJantke, Dominik
dc.contributor.authorMarziale, Alexander N.
dc.contributor.authorReiner, Thomas
dc.contributor.authorKraus, Florian
dc.contributor.authorHerdtweck, Eberhardt
dc.contributor.authorRaba, Andreas
dc.contributor.authorEppinger, Jörg
dc.date.accessioned2015-08-03T11:34:56Z
dc.date.available2015-08-03T11:34:56Z
dc.date.issued2013-11
dc.identifier.issn0022328X
dc.identifier.doi10.1016/j.jorganchem.2013.05.027
dc.identifier.urihttp://hdl.handle.net/10754/563063
dc.description.abstractSite-directed conjugation of metal centers to proteins is fundamental for biological and bioinorganic applications of transition metals. However, methods for the site-selective introduction of metal centers remain scarce. Herein, we present broadly applicable synthetic strategies for the conjugation of bioactive molecules with a range of organometallic complexes. Following three different synthetic strategies, we were able to synthesize a small library of metal conjugated protein markers featuring different types of protein reactive sites (epoxides, phenylphosphonates, fluorosulfonates and fluorophosphonate groups) as well as different late transition metals (iron, ruthenium, rhodium, palladium and platinum). The products were isolated in moderate to excellent yields and high purity. Furthermore, X-ray diffraction of the metalated protein markers corroborates structural integrity of the metal complex and the protein reactive site. © 2013 Elsevier B.V. All rights reserved.
dc.description.sponsorshipThe authors thank Ramona Lex for help with crystallization. This work was supported by KAUST-GCR (FIC/2010/07) and the KAUST baseline fund (J.E.). We also thank the Elitenetzwerk Bayern for graduate fellowships for T.R. and A.N.M.
dc.publisherElsevier BV
dc.subjectBioinorganic chemistry
dc.subjectMetalloenzymes
dc.subjectPeptide coupling
dc.subjectProtease inhibitors
dc.titleSynthetic strategies for efficient conjugation of organometallic complexes with pendant protein reactive markers
dc.typeArticle
dc.contributor.departmentKAUST Catalysis Center (KCC)
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Division
dc.contributor.departmentChemical Science Program
dc.contributor.departmentBiological & Organometallic Catalysis Laboratories
dc.identifier.journalJournal of Organometallic Chemistry
dc.contributor.institutionDepartment Chemie, Technische Universität München, Lichtenbergstr. 4, D-85747 Garching bei München, Germany
dc.contributor.institutionDepartment of Radiology, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, United States
kaust.personEppinger, Jörg
kaust.personJantke, Dominik
kaust.personMarziale, Alexander N.
kaust.personReiner, Thomas
dc.relation.isSupplementedByJantke, D., Marziale, A. N., Reiner, T., Kraus, F., Herdtweck, E., Raba, A., & Eppinger, J. (2013). CCDC 929468: Experimental Crystal Structure Determination [Data set]. Cambridge Crystallographic Data Centre. https://doi.org/10.5517/cc1065v7
dc.relation.isSupplementedByDOI:10.5517/cc1065v7
dc.relation.isSupplementedByHANDLE:http://hdl.handle.net/10754/624198
dc.relation.isSupplementedByJantke, D., Marziale, A. N., Reiner, T., Kraus, F., Herdtweck, E., Raba, A., & Eppinger, J. (2013). CCDC 868620: Experimental Crystal Structure Determination [Data set]. Cambridge Crystallographic Data Centre. https://doi.org/10.5517/ccy4w0y
dc.relation.isSupplementedByDOI:10.5517/ccy4w0y
dc.relation.isSupplementedByHANDLE:http://hdl.handle.net/10754/624685


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