Synthetic strategies for efficient conjugation of organometallic complexes with pendant protein reactive markers
Type
ArticleAuthors
Jantke, DominikMarziale, Alexander N.
Reiner, Thomas

Kraus, Florian
Herdtweck, Eberhardt
Raba, Andreas
Eppinger, Jörg

KAUST Department
Biological & Organometallic Catalysis LaboratoriesChemical Science Program
KAUST Catalysis Center (KCC)
Physical Science and Engineering (PSE) Division
KAUST Grant Number
FIC/2010/07Date
2013-11Permanent link to this record
http://hdl.handle.net/10754/563063
Metadata
Show full item recordAbstract
Site-directed conjugation of metal centers to proteins is fundamental for biological and bioinorganic applications of transition metals. However, methods for the site-selective introduction of metal centers remain scarce. Herein, we present broadly applicable synthetic strategies for the conjugation of bioactive molecules with a range of organometallic complexes. Following three different synthetic strategies, we were able to synthesize a small library of metal conjugated protein markers featuring different types of protein reactive sites (epoxides, phenylphosphonates, fluorosulfonates and fluorophosphonate groups) as well as different late transition metals (iron, ruthenium, rhodium, palladium and platinum). The products were isolated in moderate to excellent yields and high purity. Furthermore, X-ray diffraction of the metalated protein markers corroborates structural integrity of the metal complex and the protein reactive site. © 2013 Elsevier B.V. All rights reserved.Citation
Jantke, D., Marziale, A. N., Reiner, T., Kraus, F., Herdtweck, E., Raba, A., & Eppinger, J. (2013). Synthetic strategies for efficient conjugation of organometallic complexes with pendant protein reactive markers. Journal of Organometallic Chemistry, 744, 82–91. doi:10.1016/j.jorganchem.2013.05.027Sponsors
The authors thank Ramona Lex for help with crystallization. This work was supported by KAUST-GCR (FIC/2010/07) and the KAUST baseline fund (J.E.). We also thank the Elitenetzwerk Bayern for graduate fellowships for T.R. and A.N.M.Publisher
Elsevier BVRelations
Is Supplemented By:- [Dataset]
Jantke, D., Marziale, A. N., Reiner, T., Kraus, F., Herdtweck, E., Raba, A., & Eppinger, J. (2013). CCDC 929468: Experimental Crystal Structure Determination [Data set]. Cambridge Crystallographic Data Centre. https://doi.org/10.5517/cc1065v7. DOI: 10.5517/cc1065v7 HANDLE: 10754/624198 - [Dataset]
Jantke, D., Marziale, A. N., Reiner, T., Kraus, F., Herdtweck, E., Raba, A., & Eppinger, J. (2013). CCDC 868620: Experimental Crystal Structure Determination [Data set]. Cambridge Crystallographic Data Centre. https://doi.org/10.5517/ccy4w0y. DOI: 10.5517/ccy4w0y HANDLE: 10754/624685
ae974a485f413a2113503eed53cd6c53
10.1016/j.jorganchem.2013.05.027