Proteome analysis of functionally differentiated bovine (Bos indicus) mammary epithelial cells isolated from milk
Singh, Surender V.
Panigrahi, Aswini Kumar
Jena, Manoj Kumar
Anand, Vijay R.
Kumar, Sudarshan Suresh
Kaushik, Jai Kumar
Mishra, Bishnu Prasad
Reddy, Vanga Siva Belum
Mohanty, Ashok Kumar
Permanent link to this recordhttp://hdl.handle.net/10754/563032
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AbstractMammary gland is made up of a branching network of ducts that end in alveoli. Terminally differentiated mammary epithelial cells (MECs) constitute the innermost layer of aveoli. They are milk-secreting cuboidal cells that secrete milk proteins during lactation. Little is known about the expression profile of proteins in the metabolically active MECs during lactation or their functional role in the lactation process. In the present investigation, we have reported the proteome map of MECs in lactating cows using 2DE MALDI-TOF/TOF MS and 1D-Gel-LC-MS/MS. MECs were isolated from milk using immunomagnetic beads and confirmed by RT-PCR and Western blotting. The 1D-Gel-LC-MS/MS and 2DE-MS/MS based approaches led to identification of 431 and 134 proteins, respectively, with a total of 497 unique proteins. Proteins identified in this study were clustered into functional groups using bioinformatics tools. Pathway analysis of the identified proteins revealed 28 pathways (p < 0.05) providing evidence for involvement of various proteins in lactation function. This study further provides experimental evidence for the presence of many proteins that have been predicted in annotated bovine genome. The data generated further provide a set of bovine MEC-specific proteins that will help the researchers to understand the molecular events taking place during lactation. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
SponsorsThe research was supported by National Agriculture Innovation Project (component 4), Indian Council of Agricultural Research (ICAR), Government of India. A part of the MS data was collected at Seattle Biomed, Seattle, USA. J.J. received a junior research fellowship from the council of scientific research (CSIR), Government of India. We acknowledge the use of computational facility BTIS, sub center, NDRI.
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BATAL: The Balloon Measurement Campaigns of the Asian Tropopause Aerosol LayerVernier, J.-P.; Fairlie, T. D.; Deshler, T.; Venkat Ratnam, M.; Gadhavi, H.; Kumar, B. S.; Natarajan, M.; Pandit, A. K.; Akhil Raj, S. T.; Hemanth Kumar, A.; Jayaraman, A.; Singh, A. K.; Rastogi, N.; Sinha, P. R.; Kumar, S.; Tiwari, S.; Wegner, T.; Baker, N.; Vignelles, D.; Stenchikov, Georgiy L.; Shevchenko, I.; Smith, J.; Bedka, K.; Kesarkar, A.; Singh, V.; Bhate, J.; Ravikiran, V.; Durga Rao, M.; Ravindrababu, S.; Patel, A.; Vernier, H.; Wienhold, F. G.; Liu, H.; Knepp, T. N.; Thomason, L.; Crawford, J.; Ziemba, L.; Moore, J.; Crumeyrolle, S.; Williamson, M.; Berthet, G.; Jégou, F.; Renard, J.-B. (Bulletin of the American Meteorological Society, American Meteorological Society, 2017-12-15) [Article]We describe and show results from a series of field campaigns that used balloonborne instruments launched from India and Saudi Arabia during the summers 2014–17 to study the nature, formation, and impacts of the Asian Tropopause Aerosol Layer (ATAL). The campaign goals were to i) characterize the optical, physical, and chemical properties of the ATAL; ii) assess its impacts on water vapor and ozone; and iii) understand the role of convection in its formation. To address these objectives, we launched 68 balloons from four locations, one in Saudi Arabia and three in India, with payload weights ranging from 1.5 to 50 kg. We measured meteorological parameters; ozone; water vapor; and aerosol backscatter, concentration, volatility, and composition in the upper troposphere and lower stratosphere (UTLS) region. We found peaks in aerosol concentrations of up to 25 cm–3 for radii > 94 nm, associated with a scattering ratio at 940 nm of ∼1.9 near the cold-point tropopause. During medium-duration balloon flights near the tropopause, we collected aerosols and found, after offline ion chromatography analysis, the dominant presence of nitrate ions with a concentration of about 100 ng m–3. Deep convection was found to influence aerosol loadings 1 km above the cold-point tropopause. The Balloon Measurements of the Asian Tropopause Aerosol Layer (BATAL) project will continue for the next 3–4 years, and the results gathered will be used to formulate a future National Aeronautics and Space Administration–Indian Space Research Organisation (NASA–ISRO) airborne campaign with NASA high-altitude aircraft.
Evaluation of Upper Tropospheric Humidity in WRF Model during Indian Summer MonsoonAttada, Raju; Kumar, Prashant; Parekh, Anant; Ravi Kumar, K.; Nagaraju, C.; Chowdary, J. S.; Nagarjuna Rao, D. (Asia-Pacific Journal of Atmospheric Sciences, Springer Nature, 2019-01-25) [Article]In this work, we evaluate the upper tropospherichumidity (UTH) in a regional atmospheric model in conjunction with remote sensing observations and reanalysis products during the Indian summer monsoon (ISM). We performed continuous Weather Research and Forecast (WRF) model simulations from 1st May to 1st October for every year during 2001 to 2011 at 45 km spatial resolution. The maximum UTH zones viz. Bay of Bengal, and central and north-east Indian regions are well represented in WRF model when compared to the satellite observations and European Centre for Medium-Range Weather Forecasts (ECMWF) interim reanalysis (ERAI) during ISM season. Analyses found that ERAI exhibits higher magnitudes of UTH over the ISM region compared to that of satellite observations (Atmospheric Infrared Sounder:AIRS) and WRF. In terms of spatial distribution, WRF is in close agreement with satellite observations as compared to ERAI, is further supported by the pattern correlation coefficients. In addition to that, our analysis suggested that WRF model could simulate the seasonal evolution of the northward movement of maximum UTH band as in AIRS and ERAI. However, UTH variability over the equatorial Indian Ocean and western north Pacific (north of Madagascar region) is underestimated (overestimated) in the WRF model compared to the observations. Nevertheless, the model is able to represent high (low) UTH over the north Indian Ocean region during active (break) period, unable to capture the northward propagation of UTH well. This indicates that the model has considerable discrepancies in simulating UTH over the deep convective monsoon region during the ISM season. It is suggested that in order to improve the UTH representation in the model over the ISM region, it is essential to reduce biases over the equatorial and southern tropical regions. Thus, this study emphasized the variations in UTH at different time scales during monsoon season along with the credibility of remote sensing observations in WRF model.
Measurement of laminar burning velocity of n-pentanol + air mixtures at elevated temperatures and a skeletal kinetic modelKatoch, Amit; Alfazazi, Adamu; Sarathy, S. Mani; Chauhan, Ayush; Kumar, Rohit; Kumar, Sudarshan (Fuel, Elsevier BV, 2018-10-01) [Article]Long chain alcohols are potential fuels for engine applications, however, their combustion characteristics need to be adequately investigated compared to short chain alcohols (C1–C4), especially at high mixture temperatures, and other conditions relevant to engine temperatures. In the present work, meso-scale diverging channel method has been used to measure the laminar burning velocity of n-pentanol + air mixtures at elevated temperatures due to existence of very limited data at higher mixture temperatures (∼473 K). The present experiments are carried out at atmospheric pressure with unburnt mixture temperature varying up to 560 K. The dependence of laminar burning velocity on temperature was correlated using the power law: , where α is the temperature exponent. The results show the existence of a minimum value of α for slightly rich mixtures. A reduced kinetic model based on the previous detailed kinetic model of Sarathy (2014) for C1–C5 straight-chain alcohols was generated with 199 species and 1427 reactions. Experimental results of laminar burning velocity of n-pentanol + air mixtures at high temperatures were compared with the present model and other kinetic models from the literature. The skeletal model accurately reproduces the measurements at various conditions.