Proteome analysis of functionally differentiated bovine (Bos indicus) mammary epithelial cells isolated from milk
Singh, Surender V.
Panigrahi, Aswini Kumar
Jena, Manoj Kumar
Anand, Vijay R.
Kumar, Sudarshan Suresh
Kaushik, Jai Kumar
Mishra, Bishnu Prasad
Reddy, Vanga Siva Belum
Mohanty, Ashok Kumar
Permanent link to this recordhttp://hdl.handle.net/10754/563032
MetadataShow full item record
AbstractMammary gland is made up of a branching network of ducts that end in alveoli. Terminally differentiated mammary epithelial cells (MECs) constitute the innermost layer of aveoli. They are milk-secreting cuboidal cells that secrete milk proteins during lactation. Little is known about the expression profile of proteins in the metabolically active MECs during lactation or their functional role in the lactation process. In the present investigation, we have reported the proteome map of MECs in lactating cows using 2DE MALDI-TOF/TOF MS and 1D-Gel-LC-MS/MS. MECs were isolated from milk using immunomagnetic beads and confirmed by RT-PCR and Western blotting. The 1D-Gel-LC-MS/MS and 2DE-MS/MS based approaches led to identification of 431 and 134 proteins, respectively, with a total of 497 unique proteins. Proteins identified in this study were clustered into functional groups using bioinformatics tools. Pathway analysis of the identified proteins revealed 28 pathways (p < 0.05) providing evidence for involvement of various proteins in lactation function. This study further provides experimental evidence for the presence of many proteins that have been predicted in annotated bovine genome. The data generated further provide a set of bovine MEC-specific proteins that will help the researchers to understand the molecular events taking place during lactation. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
SponsorsThe research was supported by National Agriculture Innovation Project (component 4), Indian Council of Agricultural Research (ICAR), Government of India. A part of the MS data was collected at Seattle Biomed, Seattle, USA. J.J. received a junior research fellowship from the council of scientific research (CSIR), Government of India. We acknowledge the use of computational facility BTIS, sub center, NDRI.
- Proteomic Analysis of Isolated Plasma Membrane Fractions from the Mammary Gland in Lactating Cows.
- Authors: Yan Q, Tang S, Tan Z, Han X, Zhou C, Kang J, Wang M
- Issue date: 2015 Aug 26
- DIGE based proteome analysis of mammary gland tissue in water buffalo (Bubalus bubalis): lactating vis-a-vis heifer.
- Authors: Jena MK, Janjanam J, Naru J, Kumar S, Kumar S, Singh S, Mohapatra SK, Kola S, Anand V, Jaswal S, Verma AK, Malakar D, Dang AK, Kaushik JK, Reddy VS, Mohanty AK
- Issue date: 2015 Apr 24
- Gene expression analysis of protein synthesis pathways in bovine mammary epithelial cells purified from milk during lactation and short-term restricted feeding.
- Authors: Sigl T, Meyer HH, Wiedemann S
- Issue date: 2014 Feb
- Milking and feed restriction regulate transcripts of mammary epithelial cells purified from milk.
- Authors: Boutinaud M, Ben Chedly MH, Delamaire E, Guinard-Flament J
- Issue date: 2008 Mar
- Evaluation of a replacement method for mammary gland biopsies by comparing gene expression in udder tissue and mammary epithelial cells isolated from milk.
- Authors: Krappmann K, Weikard R, Kühn C
- Issue date: 2012 Oct
Showing items related by title, author, creator and subject.
TIN OXIDE MODIFIED SILICON NANOWIRES ON SILICON CHIP AS AMMONIA SENSORYuvaraja, Saravanan; Dhasmana, Hrishikesh; Kumar, Amit; Kumar, Vivek; Verma, Abhishek; Kumar Jain, Vinod; Nabil Salama, Khaled (2019-01-13) [Poster]Title: TIN OXIDE MODIFIED SILICON NANOWIRES ON SILICON CHIP AS AMMONIA SENSOR Authors: Saravanan Yuvaraja1,2, Hrishikesh Dhasmana2, Amit Kumar2, Vivek Kumar2, Abhishek Verma2, V. K. Jain2 and Khaled Salama1. Section 1: HOW YOU ARE EXPOSED TO AMMONIA ? Section 2: HOW AMMONIA AFFECTS YOUR HEALTH ? Section 3: WE CAN SAVE YOU FROM AMMONIA EXPOSURE Level 1: Synthesis Level 2: Performance analysis Level 3: Prototype demonstration Attention: Both Synthesis process flow and Gas sensing mechanism concept of the reported device architecture is presented in the video
BATAL: The Balloon Measurement Campaigns of the Asian Tropopause Aerosol LayerVernier, J.-P.; Fairlie, T. D.; Deshler, T.; Venkat Ratnam, M.; Gadhavi, H.; Kumar, B. S.; Natarajan, M.; Pandit, A. K.; Akhil Raj, S. T.; Hemanth Kumar, A.; Jayaraman, A.; Singh, A. K.; Rastogi, N.; Sinha, P. R.; Kumar, S.; Tiwari, S.; Wegner, T.; Baker, N.; Vignelles, D.; Stenchikov, Georgiy L.; Shevchenko, Illia; Smith, J.; Bedka, K.; Kesarkar, A.; Singh, V.; Bhate, J.; Ravikiran, V.; Durga Rao, M.; Ravindrababu, S.; Patel, A.; Vernier, H.; Wienhold, F. G.; Liu, H.; Knepp, T. N.; Thomason, L.; Crawford, J.; Ziemba, L.; Moore, J.; Crumeyrolle, S.; Williamson, M.; Berthet, G.; Jégou, F.; Renard, J.-B. (Bulletin of the American Meteorological Society, American Meteorological Society, 2017-12-15) [Article]We describe and show results from a series of field campaigns that used balloonborne instruments launched from India and Saudi Arabia during the summers 2014–17 to study the nature, formation, and impacts of the Asian Tropopause Aerosol Layer (ATAL). The campaign goals were to i) characterize the optical, physical, and chemical properties of the ATAL; ii) assess its impacts on water vapor and ozone; and iii) understand the role of convection in its formation. To address these objectives, we launched 68 balloons from four locations, one in Saudi Arabia and three in India, with payload weights ranging from 1.5 to 50 kg. We measured meteorological parameters; ozone; water vapor; and aerosol backscatter, concentration, volatility, and composition in the upper troposphere and lower stratosphere (UTLS) region. We found peaks in aerosol concentrations of up to 25 cm–3 for radii > 94 nm, associated with a scattering ratio at 940 nm of ∼1.9 near the cold-point tropopause. During medium-duration balloon flights near the tropopause, we collected aerosols and found, after offline ion chromatography analysis, the dominant presence of nitrate ions with a concentration of about 100 ng m–3. Deep convection was found to influence aerosol loadings 1 km above the cold-point tropopause. The Balloon Measurements of the Asian Tropopause Aerosol Layer (BATAL) project will continue for the next 3–4 years, and the results gathered will be used to formulate a future National Aeronautics and Space Administration–Indian Space Research Organisation (NASA–ISRO) airborne campaign with NASA high-altitude aircraft.
Synthesis of novel 1H-1,2,3-triazole tethered C-5 substituted uracil-isatin conjugates and their cytotoxic evaluationKumar, Kewal; Sagar, Sunil; Esau, Luke; Kaur, Mandeep; Kumar, Vipan (European Journal of Medicinal Chemistry, Elsevier BV, 2012-12) [Article]The present manuscript describes the synthesis of uracil-isatin hybrids via azide-alkyne cycloadditions and their cytotoxic evaluation against three human cancer cell lines viz. HeLa (cervix), MCF-7 (breast) and DU145 (prostate) using MTT assay. The evaluation studies revealed the dependence of cytotoxicity on C-5 substituents of both uracil and isatin as well as the alkyl chain length with compounds 6g and 6k showing IC50 values 18.21 and 13.90 μM respectively against DU145 cell lines. Most of the synthesized conjugates exhibited considerable selectivity against MCF-7 and DU145 cell lines. © 2012 Elsevier Masson SAS. All rights reserved.