Show simple item record

dc.contributor.authorPetit, Jean Marie
dc.contributor.authorGyger, Joël
dc.contributor.authorBurlet-Godinot, Sophie
dc.contributor.authorFiumelli, Hubert
dc.contributor.authorMartin, Jean Luc
dc.contributor.authorMagistretti, Pierre J.
dc.date.accessioned2015-08-03T11:34:06Z
dc.date.available2015-08-03T11:34:06Z
dc.date.issued2013-10-01
dc.identifier.issn01618105
dc.identifier.pmid24082304
dc.identifier.doi10.5665/sleep.3034
dc.identifier.urihttp://hdl.handle.net/10754/563029
dc.description.abstractStudy Objectives: There is growing evidence indicating that in order to meet the neuronal energy demands, astrocytes provide lactate as an energy substrate for neurons through a mechanism called "astrocyte-neuron lactate shuttle" (ANLS). Since neuronal activity changes dramatically during vigilance states, we hypothesized that the ANLS may be regulated during the sleep-wake cycle. To test this hypothesis we investigated the expression of genes associated with the ANLS specifcally in astrocytes following sleep deprivation. Astrocytes were purifed by fuorescence-activated cell sorting from transgenic mice expressing the green fuorescent protein (GFP) under the control of the human astrocytic GFAP-promoter. Design: 6-hour instrumental sleep deprivation (TSD). Setting: Animal sleep research laboratory. Participants: Young (P23-P27) FVB/N-Tg (GFAP-GFP) 14Mes/J (Tg) mice of both sexes and 7-8 week male Tg and FVB/Nj mice. Interventions: Basal sleep recordings and sleep deprivation achieved using a modifed cage where animals were gently forced to move. Measurements and Results: Since Tg and FVB/Nj mice displayed a similar sleep-wake pattern, we performed a TSD in young Tg mice. Total RNA was extracted from the GFP-positive and GFP-negative cells sorted from cerebral cortex. Quantitative RT-PCR analysis showed that levels of Glut1, a-2-Na/K pump, Glt1, and Ldha mRNAs were signifcantly increased following TSD in GFP-positive cells. In GFP-negative cells, a tendency to increase, although not signifcant, was observed for Ldha, Mct2, and α-3-Na/K pump mRNAs. Conclusions: This study shows that TSD induces the expression of genes associated with ANLS specifcally in astrocytes, underlying the important role of astrocytes in the maintenance of the neuro-metabolic coupling across the sleep-wake cycle.
dc.description.sponsorshipThe authors warmly thank M. Garcia, G. Tapia, and S. Winkler of the Flow Cytometry Core Facility (Life Sciences Faculty, EPFL, Lausanne, Switzerland) for their technical expertise. This work was supported by a Swiss National Science Foundation grant (3100AO-108336/1) to Dr. Magistretti.
dc.publisherAssociated Professional Sleep Societies (APSS)
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3773194
dc.subjectEnergy metabolism
dc.subjectFow cytometry
dc.subjectGlia
dc.subjectGlucose
dc.subjectGlutamate
dc.subjectTransgenic mice
dc.titleGenes involved in the astrocyte-neuron lactate shuttle (ANLS) are specifcally regulated in cortical astrocytes following sleep deprivation in mice
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.identifier.journalSLEEP
dc.identifier.pmcidPMC3773194
dc.contributor.institutionLNDC-BMI-SV, EPFL, Life Sciences Faculty, Swiss Federal Institute of Technology, Station 19, 1015 Lausanne, Switzerland
dc.contributor.institutionCenter for Psychiatric Neuroscience, Department of Psychiatry, CHUV, Prilly, Switzerland
kaust.personMagistretti, Pierre J.


This item appears in the following Collection(s)

Show simple item record