Noninvasive microelectrode ion flux estimation technique (MIFE) for the study of the regulation of root membrane transport by cyclic nucleotides
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Molecular Signalling Group
Permanent link to this recordhttp://hdl.handle.net/10754/562968
MetadataShow full item record
AbstractChanges in ion permeability and subsequently intracellular ion concentrations play a crucial role in intracellular and intercellular communication and, as such, confer a broad array of developmental and adaptive responses in plants. These changes are mediated by the activity of plasma-membrane based transport proteins many of which are controlled by cyclic nucleotides and/or other signaling molecules. The MIFE technique for noninvasive microelectrode ion flux measuring allows concurrent quantification of net fluxes of several ions with high spatial (μm range) and temporal (ca. 5 s) resolution, making it a powerful tool to study various aspects of downstream signaling events in plant cells. This chapter details basic protocols enabling the application of the MIFE technique to study regulation of root membrane transport in general and cyclic nucleotide mediated transport in particular. © Springer Science+Business Media New York 2013.
JournalMethods in Molecular Biology
- Ion flux measurements using the MIFE technique.
- Authors: Shabala S, Shabala L, Bose J, Cuin T, Newman I
- Issue date: 2013
- Quantifying kinetics of net ion fluxes from plant tissues by non-invasive microelectrode measuring MIFE technique.
- Authors: Shabala S, Cuin TA, Shabala L, Newman I
- Issue date: 2012
- Na(+)/H(+) antiporter activity of the SOS1 gene: lifetime imaging analysis and electrophysiological studies on Arabidopsis seedlings.
- Authors: Guo KM, Babourina O, Rengel Z
- Issue date: 2009 Oct
- Plant cell growth and ion flux responses to the streptomycete phytotoxin thaxtomin A: calcium and hydrogen flux patterns revealed by the non-invasive MIFE technique.
- Authors: Tegg RS, Melian L, Wilson CR, Shabala S
- Issue date: 2005 Apr
- Detection of reactive oxygen species downstream of cyclic nucleotide signals in plants.
- Authors: Walker RK, Berkowitz GA
- Issue date: 2013