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    Noninvasive microelectrode ion flux estimation technique (MIFE) for the study of the regulation of root membrane transport by cyclic nucleotides

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    Type
    Article
    Authors
    Ordoñez, Natalia Maria
    Shabala, Lana
    Gehring, Christoph A cc
    Shabala, Sergey Nikolayevich
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Molecular Signalling Group
    Date
    2013-09-03
    Permanent link to this record
    http://hdl.handle.net/10754/562968
    
    Metadata
    Show full item record
    Abstract
    Changes in ion permeability and subsequently intracellular ion concentrations play a crucial role in intracellular and intercellular communication and, as such, confer a broad array of developmental and adaptive responses in plants. These changes are mediated by the activity of plasma-membrane based transport proteins many of which are controlled by cyclic nucleotides and/or other signaling molecules. The MIFE technique for noninvasive microelectrode ion flux measuring allows concurrent quantification of net fluxes of several ions with high spatial (μm range) and temporal (ca. 5 s) resolution, making it a powerful tool to study various aspects of downstream signaling events in plant cells. This chapter details basic protocols enabling the application of the MIFE technique to study regulation of root membrane transport in general and cyclic nucleotide mediated transport in particular. © Springer Science+Business Media New York 2013.
    Publisher
    Humana Press
    Journal
    Methods in Molecular Biology
    ISBN
    9781627034401
    DOI
    10.1007/978-1-62703-441-8-7
    PubMed ID
    23681574
    ae974a485f413a2113503eed53cd6c53
    10.1007/978-1-62703-441-8-7
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program

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