Computational identification of candidate nucleotide cyclases in higher plants
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Molecular Signalling Group
Permanent link to this recordhttp://hdl.handle.net/10754/562967
MetadataShow full item record
AbstractIn higher plants guanylyl cyclases (GCs) and adenylyl cyclases (ACs) cannot be identified using BLAST homology searches based on annotated cyclic nucleotide cyclases (CNCs) of prokaryotes, lower eukaryotes, or animals. The reason is that CNCs are often part of complex multifunctional proteins with different domain organizations and biological functions that are not conserved in higher plants. For this reason, we have developed CNC search strategies based on functionally conserved amino acids in the catalytic center of annotated and/or experimentally confirmed CNCs. Here we detail this method which has led to the identification of >25 novel candidate CNCs in Arabidopsis thaliana, several of which have been experimentally confirmed in vitro and in vivo. We foresee that the application of this method can be used to identify many more members of the growing family of CNCs in higher plants. © Springer Science+Business Media New York 2013.
JournalMethods in Molecular Biology
- The Arabidopsis thaliana proteome harbors undiscovered multi-domain molecules with functional guanylyl cyclase catalytic centers.
- Authors: Wong A, Gehring C
- Issue date: 2013 Jul 8
- Recombinant expression and functional testing of candidate adenylate cyclase domains.
- Authors: Ruzvidzo O, Dikobe BT, Kawadza DT, Mabadahanye GH, Chatukuta P, Kwezi L
- Issue date: 2013
- Identification of a novel Arabidopsis thaliana nitric oxide-binding molecule with guanylate cyclase activity in vitro.
- Authors: Mulaudzi T, Ludidi N, Ruzvidzo O, Morse M, Hendricks N, Iwuoha E, Gehring C
- Issue date: 2011 Sep 2
- Identification of a novel protein with guanylyl cyclase activity in Arabidopsis thaliana.
- Authors: Ludidi N, Gehring C
- Issue date: 2003 Feb 21
- The evolution of guanylyl cyclases as multidomain proteins: conserved features of kinase-cyclase domain fusions.
- Authors: Biswas KH, Shenoy AR, Dutta A, Visweswariah SS
- Issue date: 2009 Jun