Roles of singlet oxygen and triplet excited state of dissolved organic matter formed by different organic matters in bacteriophage MS2 inactivation
AuthorsRosado-Lausell, Sahid L.
Gutiérrez, Leonardo A.
Romero-Maraccini, Ofelia C.
Nguyen, Thanh Ha
KAUST DepartmentEnvironmental Science and Engineering Program
Biological and Environmental Sciences and Engineering (BESE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/562940
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AbstractInactivation of bacteriophage MS2 by reactive oxygen species (ROS) and triplet excited state of dissolved organic matter (3DOM*) produced by irradiation of natural and synthetic sensitizers with simulated sunlight of wavelengths greater than 320nm was investigated. Natural sensitizers included purified DOM isolates obtained from wastewater and river waters, and water samples collected from Singapore River, Stamford Canal, and Marina Bay Reservoir in Singapore. Linear correlations were found between MS2 inactivation rate constants (kobs) and the photo-induced reaction rate constants of 2,4,6-trimethylphenol (TMP), a probe compound shown to react mainly with 3DOM*. Linear correlations between MS2 kobs and singlet oxygen (1O2) concentrations were also found for both purified DOM isolates and natural water samples. These correlations, along with data from quenching experiments and experiments with synthetic sensitizers, Rose Bengal (RB), 3'-methoxyacetophenone (3'-MAP), and nitrite (NO2-), suggest that 1O2, 3DOM*, and hydroxyl radicals (•OH) could inactivate bacteriophage MS2. Linear correlations between MS2 kobs and Specific Ultraviolet Absorption determined at 254nm (SUVA254) were also found for both purified DOM isolates and natural samples. These results suggest the potential use of TMP as a chemical probe and SUVA254 as an indicator for virus inactivation in natural and purified DOM water samples. © 2013 Elsevier Ltd.
SponsorsWe acknowledge the financial support of the Academic Excellence Alliance (AEA) program at King Abdullah University of Science and Technology (KAUST), NSF 1066152, Safe Global Water Institute at University of Illinois, NSF EAPSI OISE-1209310, and NSF CAREER grants. Ee Ling Yong, Gan Jie, Chenxi Sun, and Tung Nguyen (National University of Singapore) are acknowledged for helping with experiments conducted in Singapore.
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