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    Split photosystem protein, linear-mapping topology, and growth of structural complexity in the plastid genome of chromera velia

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    Type
    Article
    Authors
    Janouškovec, Jan
    Sobotka, Roman
    Lai, Dehua
    Flegontov, Pavel N.
    Koník, Peter
    Komenda, Josef
    Ali, Shahjahan
    Prášil, Ondřej
    Pain, Arnab cc
    Oborník, Miroslav
    Lukeš, Juliuš
    Keeling, Patrick J J.
    KAUST Department
    Bioscience Core Lab
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Computational Bioscience Research Center (CBRC)
    Core Labs
    Pathogen Genomics Laboratory
    KAUST Grant Number
    IC/2010/09
    Date
    2013-08-22
    Online Publication Date
    2013-08-22
    Print Publication Date
    2013-11
    Permanent link to this record
    http://hdl.handle.net/10754/562917
    
    Metadata
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    Abstract
    The canonical photosynthetic plastid genomes consist of a single circular-mapping chromosome that encodes a highly conserved protein core, involved in photosynthesis and ATP generation. Here, we demonstrate that the plastid genome of the photosynthetic relative of apicomplexans, Chromera velia, departs from this view in several unique ways. Core photosynthesis proteins PsaA and AtpB have been broken into two fragments, which we show are independently transcribed, oligoU-tailed, translated, and assembled into functional photosystem I and ATP synthase complexes. Genome-wide transcription profiles support expression of many other highly modified proteins, including several that contain extensions amounting to hundreds of amino acids in length. Canonical gene clusters and operons have been fragmented and reshuffled into novel putative transcriptional units. Massive genomic coverage by paired-end reads, coupled with pulsed-field gel electrophoresis and polymerase chain reaction, consistently indicate that the C. velia plastid genome is linear-mapping, a unique state among all plastids. Abundant intragenomic duplication probably mediated by recombination can explain protein splits, extensions, and genome linearization and is perhaps the key driving force behind the many features that defy the conventional ways of plastid genome architecture and function. © The Author 2013.
    Citation
    Janouškovec, J., Sobotka, R., Lai, D.-H., Flegontov, P., Koník, P., Komenda, J., … Keeling, P. J. (2013). Split Photosystem Protein, Linear-Mapping Topology, and Growth of Structural Complexity in the Plastid Genome of Chromera velia. Molecular Biology and Evolution, 30(11), 2447–2462. doi:10.1093/molbev/mst144
    Sponsors
    This work was supported by a grant from the Canadian Institutes of Health Research to P.J.K. (MOP-42517); by the Czech Science Foundation projects P506/12/1522 and P501/12/G055 to M.O.; by the Praemium Academiae award to J.L.; by Award IC/2010/09 by the King Abdullah University of Science and Technology (KAUST) to A. P., M.O., and J.L.; and by the project Algatech (CZ.1.05/2.1.00/03.0110) to R. S., J.K., and O.P.. P.J.K. and J.L. are Fellows of the Canadian Institute for Advanced Research. P.J.K. was supported by a Fellowship from the John Simon Guggenheim Foundation.
    Publisher
    Oxford University Press (OUP)
    Journal
    Molecular Biology and Evolution
    DOI
    10.1093/molbev/mst144
    PubMed ID
    23974208
    ae974a485f413a2113503eed53cd6c53
    10.1093/molbev/mst144
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Computational Bioscience Research Center (CBRC); Bioscience Core Lab

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