Characterization and DNA-binding specificities of Ralstonia TAL-like effectors
Type
ArticleAuthors
Li, LixinAtef, Ahmed
Piatek, Agnieszka Anna

Ali, Zahir

Piatek, Marek J.

Aouida, Mustapha

Sharakuu, Altanbadralt
Mahjoub, Ali

Wang, Guangchao
Khan, Mohammad Suhail
Fedoroff, Nina V.

Zhu, Jian-Kang

Mahfouz, Magdy M.

KAUST Department
Advanced Nanofabrication, Imaging and Characterization Core LabBiological and Environmental Science and Engineering (BESE) Division
Bioscience Program
Center for Desert Agriculture
Core Labs
Imaging and Characterization Core Lab
Laboratory for Genome Engineering
Plant Science
Plant Science Program
Date
2013-07Permanent link to this record
http://hdl.handle.net/10754/562847
Metadata
Show full item recordAbstract
Transcription activator-like effectors (TALEs) from Xanthomonas sp. have been used as customizable DNA-binding modules for genome-engineering applications. Ralstonia solanacearum TALE-like proteins (RTLs) exhibit similar structural features to TALEs, including a central DNA-binding domain composed of 35 amino acid-long repeats. Here, we characterize the RTLs and show that they localize in the plant cell nucleus, mediate DNA binding, and might function as transcriptional activators. RTLs have a unique DNA-binding architecture and are enriched in repeat variable di-residues (RVDs), which determine repeat DNA-binding specificities. We determined the DNA-binding specificities for the RVD sequences ND, HN, NP, and NT. The RVD ND mediates highly specific interactions with C nucleotide, HN interacts specifically with A and G nucleotides, and NP binds to C, A, and G nucleotides. Moreover, we developed a highly efficient repeat assembly approach for engineering RTL effectors. Taken together, our data demonstrate that RTLs are unique DNA-targeting modules that are excellent alternatives to be tailored to bind to user-selected DNA sequences for targeted genomic and epigenomic modifications. These findings will facilitate research concerning RTL molecular biology and RTL roles in the pathogenicity of Ralstonia spp. © 2013 The Author.Citation
Li, L., Atef, A., Piatek, A., Ali, Z., Piatek, M., Aouida, M., … Mahfouz, M. M. (2013). Characterization and DNA-Binding Specificities of Ralstonia TAL-Like Effectors. Molecular Plant, 6(4), 1318–1330. doi:10.1093/mp/sst006Sponsors
This research is funded from the Center for Desert Agriculture baseline funding. The work in J.-K.Z.'s lab is supported by the National Institute of Health grant R01GM070795.Publisher
Elsevier BVJournal
Molecular PlantPubMed ID
23300258PubMed Central ID
PMC3716395Additional Links
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3716395ae974a485f413a2113503eed53cd6c53
10.1093/mp/sst006
Scopus Count
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