Molecular cloning, sequencing and structural studies of granulocyte-macrophage colony-stimulating factor (GM-CSF) from Indian water buffalo (Bubalus bubalis)

Type
Article

Authors
Sugumar, Thennarasu
Ganesan, Pugalenthi
Harishankar, Murugesan
Dhinakar Raj, Gopal

KAUST Department
Bioscience Core Lab
Core Labs

Online Publication Date
2013-06-25

Print Publication Date
2014-02

Date
2013-06-25

Abstract
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that is essential for growth and development of progenitors of granulocytes and monocytes/macrophages. In this study, we report molecular cloning, sequencing and characterization of GM-CSF from Indian water buffalo, Bubalus bubalis. In addition, we performed sequence and structural analysis for buffalo GM-CSF. Buffalo GM-CSF has been compared with 17 mammalian GM-CSFs using multiple sequence alignment and phylogenetic tree. Three-dimensional model for buffalo GM-CSF and human receptor complex was built using homology modelling to study cross-reactivity between two species. Detailed analysis was performed to study GM-CSF interface and various interactions at the interface. © 2013 John Wiley & Sons Ltd.

Citation
Sugumar, T., Pugalenthi, G., Harishankar, M., & Dhinakar Raj, G. (2013). Molecular cloning, sequencing and structural studies of granulocyte-macrophage colony-stimulating factor (GM-CSF) from Indian water buffalo (Bubalus bubalis). International Journal of Immunogenetics, 41(1), 74–80. doi:10.1111/iji.12074

Acknowledgements
The authors thank the Department of Biotechnology (DBT), Government of India, for the financial assistance to this Project. ST and MHS were SRFs who worked in this project. GP carried out the bioinformatics analysis.

Publisher
Wiley

Journal
International Journal of Immunogenetics

DOI
10.1111/iji.12074

PubMed ID
23800159

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