Microbial community evolution during simulated managed aquifer recharge in response to different biodegradable dissolved organic carbon (BDOC) concentrations
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Environmental Biotechnology Research Group
Environmental Science and Engineering Program
Water Desalination and Reuse Research Center (WDRC)
Permanent link to this recordhttp://hdl.handle.net/10754/562739
MetadataShow full item record
AbstractThis study investigates the evolution of the microbial community in laboratory-scale soil columns simulating the infiltration zone of managed aquifer recharge (MAR) systems and analogous natural aquifer sediment ecosystems. Parallel systems were supplemented with either moderate (1.1 mg/L) or low (0.5 mg/L) biodegradable dissolved organic carbon (BDOC) for a period of six months during which time, spatial (1 cm, 30 cm, 60 cm, 90 cm, and 120 cm) and temporal (monthly) analyses of sediment-associated microbial community structure were analyzed. Total microbial biomass associated with sediments was positively correlated with BDOC concentration where a significant decline in BDOC was observed along the column length. Analysis of 16S rRNA genes indicated dominance by Bacteria with Archaea comprising less than 1 percent of the total community. Proteobacteria was found to be the major phylum in samples from all column depths with contributions from Betaproteobacteria, Alphaproteobacteria and Gammaproteobacteria. Microbial community structure at all the phylum, class and genus levels differed significantly at 1 cm between columns receiving moderate and low BDOC concentrations; in contrast strong similarities were observed both between parallel column systems and across samples from 30 to 120 cm depths. Samples from 1 cm depth of the low BDOC columns exhibited higher microbial diversity (expressed as Shannon Index) than those at 1 cm of moderate BDOC columns, and both increased from 5.4 to 5.9 at 1 cm depth to 6.7-8.3 at 30-120 cm depths. The microbial community structure reached steady state after 3-4 months since the initiation of the experiment, which also resulted in an improved DOC removal during the same time period. This study suggested that BDOC could significantly influence microbial community structure regarding both composition and diversity of artificial MAR systems and analogous natural aquifer sediment ecosystems. © 2013 Elsevier Ltd.
SponsorsThis research was supported by discretionary investigator funds (J.D., P.S.) at King Abdullah University of Science and Technology (KAUST). The material presented is also based in part upon work supported by the National Science Foundation under Grant No. CBET-1055396 (J.S.) and Cooperative Agreement EEC-1028968 (J.D. and J.S.). The authors are thankful for technical assistance provided by Shahjahan Ali and Mohammed S Alarawi at the Biosciences Core Laboratory, King Abdullah University of Science and Technology.
- The role of microbial adaptation and biodegradable dissolved organic carbon on the attenuation of trace organic chemicals during groundwater recharge.
- Authors: Hoppe-Jones C, Dickenson ER, Drewes JE
- Issue date: 2012 Oct 15
- Dissolved organic carbon influences microbial community composition and diversity in managed aquifer recharge systems.
- Authors: Li D, Sharp JO, Saikaly PE, Ali S, Alidina M, Alarawi MS, Keller S, Hoppe-Jones C, Drewes JE
- Issue date: 2012 Oct
- Investigating the role for adaptation of the microbial community to transform trace organic chemicals during managed aquifer recharge.
- Authors: Alidina M, Li D, Drewes JE
- Issue date: 2014 Jun 1
- Role of primary substrate composition on microbial community structure and function and trace organic chemical attenuation in managed aquifer recharge systems.
- Authors: Li D, Alidina M, Drewes JE
- Issue date: 2014 Jun
- Spatial and temporal changes in microbial community structure associated with recharge-influenced chemical gradients in a contaminated aquifer.
- Authors: Haack SK, Fogarty LR, West TG, Alm EW, McGuire JT, Long DT, Hyndman DW, Forney LJ
- Issue date: 2004 May