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dc.contributor.authorSabelli, Paolo A.
dc.contributor.authorLiu, Yan
dc.contributor.authorDante, Ricardo Augusto
dc.contributor.authorLizarraga, Lucina E.
dc.contributor.authorNguyen, Hong N.
dc.contributor.authorBrown, Sara W.
dc.contributor.authorKlingler, John
dc.contributor.authorYu, Jingjuan
dc.contributor.authorLaBrant, Evan
dc.contributor.authorLayton, Tracy M.
dc.contributor.authorFeldman, Max
dc.contributor.authorLarkins, Brian A.
dc.date.accessioned2015-08-03T11:03:15Z
dc.date.available2015-08-03T11:03:15Z
dc.date.issued2013-04-22
dc.identifier.citationSabelli, P. A., Liu, Y., Dante, R. A., Lizarraga, L. E., Nguyen, H. N., Brown, S. W., … Larkins, B. A. (2013). Control of cell proliferation, endoreduplication, cell size, and cell death by the retinoblastoma-related pathway in maize endosperm. Proceedings of the National Academy of Sciences, 110(19), E1827–E1836. doi:10.1073/pnas.1304903110
dc.identifier.issn00278424
dc.identifier.pmid23610440
dc.identifier.doi10.1073/pnas.1304903110
dc.identifier.urihttp://hdl.handle.net/10754/562723
dc.description.abstractThe endospermof cereal grains is one of the most valuable products of modern agriculture. Cereal endosperm development comprises different phases characterized by mitotic cell proliferation, endoreduplication, the accumulation of storage compounds, and programmed cell death. Although manipulation of these processes could maximize grain yield, how they are regulated and integrated is poorly understood. We show that the Retinoblastoma-related (RBR) pathway controls key aspects of endosperm development in maize. Down-regulation of RBR1 by RNAi resulted in up-regulation of RBR3-type genes, as well as the MINICHROMOSOME MAINTENANCE 2-7 gene family and PROLIFERATING CELL NUCLEAR ANTIGEN, which encode essential DNA replication factors. Both the mitotic and endoreduplication cell cycles were stimulated. Developing transgenic endosperm contained 42-58% more cells and ~70% more DNA than wild type, whereas there was a reduction in cell and nuclear sizes. In addition, cell death was enhanced. The DNA content of mature endosperm increased 43% upon RBR1 downregulation, whereas storage protein content and kernel weight were essentially not affected. Down-regulation of both RBR1 and CYCLIN DEPENDENT KINASE A (CDKA);1 indicated that CDKA;1 is epistatic to RBR1 and controls endoreduplication through an RBR1- dependent pathway. However, the repressive activity of RBR1 on downstream targets was independent from CDKA;1, suggesting diversification of RBR1 activities. Furthermore, RBR1 negatively regulated CDK activity, suggesting the presence of a feedback loop. These results indicate that the RBR1 pathway plays a major role in regulation of different processes during maize endosperm development and suggest the presence of tissue/organlevel regulation of endosperm/seed homeostasis.
dc.publisherProceedings of the National Academy of Sciences
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651506
dc.subjectEndocycle
dc.subjectSeed development
dc.titleControl of cell proliferation, endoreduplication, cell size, and cell death by the retinoblastoma-related pathway in maize endosperm
dc.typeArticle
dc.contributor.departmentAward Monitoring&Integrative Activities
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentDesert Agriculture Initiative
dc.identifier.journalProceedings of the National Academy of Sciences of the United States of America
dc.identifier.pmcidPMC3651506
dc.contributor.institutionSchool of Plant Sciences, University of Arizona, Tucson, AZ 85721, United States
dc.contributor.institutionState Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China
dc.contributor.institutionHerman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202, United States
dc.contributor.institutionEmbrapa Agricultural Informatics, Campinas, SP 13083-886, Brazil
dc.contributor.institutionPharmacology and Toxicology Department, College of Pharmacy, University of Arizona, Tucson, AZ 85721, United States
dc.contributor.institutionDonald Danforth Plant Science Center, St. Louis, MO 63132, United States
kaust.personKlingler, John
dc.date.published-online2013-04-22
dc.date.published-print2013-05-07


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