General anaesthetics do not impair developmental expression of the KCC2 potassium-chloride cotransporter in neonatal rats during the brain growth spurt
Online Publication Date2013-03-27
Print Publication Date2013-06
Permanent link to this recordhttp://hdl.handle.net/10754/562689
MetadataShow full item record
AbstractBackgroundThe developmental transition from depolarizing to hyperpolarizing γ-aminobutyric acid-mediated neurotransmission is primarily mediated by an increase in the amount of the potassium-chloride cotransporter KCC2 during early postnatal life. However, it is not known whether early neuronal activity plays a modulatory role in the expression of total KCC2 mRNA and protein in the immature brain. As general anaesthetics are powerful modulators of neuronal activity, the purpose of this study was to explore how these drugs affect KCC2 expression during the brain growth spurt.MethodsWistar rat pups were exposed to either a single dose or 6 h of midazolam, propofol, or ketamine anaesthesia at postnatal days 0, 5, 10, or 15. KCC2 expression was assessed using immunoblotting, immunohistochemistry, or quantitative polymerase chain reaction analysis up to 3 days post-exposure in the medial prefrontal cortex.ResultsThere was a progressive and steep increase in the expression of KCC2 between birth and 2 weeks of age. Exposure to midazolam, propofol, or ketamine up to 6 h at any investigated stages of the brain growth spurt did not influence the expression of this cotransporter protein.ConclusionI.V. general anaesthetics do not seem to influence developmental expression of KCC2 during the brain growth spurt. © 2013 © The Author .
SponsorsThis work was founded by the Swiss National Science Foundation (grant 31003A-130625 to L. V.) and by the Academy of Finland and the Letten Foundation (to K.K.).
JournalBritish Journal of Anaesthesia
- Developmental stage-dependent impact of midazolam on calbindin, calretinin and parvalbumin expression in the immature rat medial prefrontal cortex during the brain growth spurt.
- Authors: Osterop SF, Virtanen MA, Loepke JR, Joseph B, Loepke AW, Vutskits L
- Issue date: 2015 Oct
- Changes of K+ -Cl- cotransporter 2 (KCC2) and circuit activity in propofol-induced impairment of long-term potentiation in rat hippocampal slices.
- Authors: Wang W, Wang H, Gong N, Xu TL
- Issue date: 2006 Oct 16
- K-Cl Cotransporter 2-mediated Cl- Extrusion Determines Developmental Stage-dependent Impact of Propofol Anesthesia on Dendritic Spines.
- Authors: Puskarjov M, Fiumelli H, Briner A, Bodogan T, Demeter K, Lacoh CM, Mavrovic M, Blaesse P, Kaila K, Vutskits L
- Issue date: 2017 May
- Developmental up-regulation of the potassium-chloride cotransporter type 2 in the rat lumbar spinal cord.
- Authors: Stil A, Liabeuf S, Jean-Xavier C, Brocard C, Viemari JC, Vinay L
- Issue date: 2009 Dec 1
- Impact of propofol anaesthesia on cytokine expression profiles in the developing rat brain: a randomised placebo-controlled experimental in-vivo study.
- Authors: Kargaran P, Lenglet S, Montecucco F, Mach F, Copin JC, Vutskits L
- Issue date: 2015 May