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    General anaesthetics do not impair developmental expression of the KCC2 potassium-chloride cotransporter in neonatal rats during the brain growth spurt

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    Type
    Article
    Authors
    Lacoh, Claudia Marvine
    Bodogan, T.
    Kaila, Kai K.
    Fiumelli, Hubert cc
    Vutskits, László
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2013-03-27
    Online Publication Date
    2013-03-27
    Print Publication Date
    2013-06
    Permanent link to this record
    http://hdl.handle.net/10754/562689
    
    Metadata
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    Abstract
    BackgroundThe developmental transition from depolarizing to hyperpolarizing γ-aminobutyric acid-mediated neurotransmission is primarily mediated by an increase in the amount of the potassium-chloride cotransporter KCC2 during early postnatal life. However, it is not known whether early neuronal activity plays a modulatory role in the expression of total KCC2 mRNA and protein in the immature brain. As general anaesthetics are powerful modulators of neuronal activity, the purpose of this study was to explore how these drugs affect KCC2 expression during the brain growth spurt.MethodsWistar rat pups were exposed to either a single dose or 6 h of midazolam, propofol, or ketamine anaesthesia at postnatal days 0, 5, 10, or 15. KCC2 expression was assessed using immunoblotting, immunohistochemistry, or quantitative polymerase chain reaction analysis up to 3 days post-exposure in the medial prefrontal cortex.ResultsThere was a progressive and steep increase in the expression of KCC2 between birth and 2 weeks of age. Exposure to midazolam, propofol, or ketamine up to 6 h at any investigated stages of the brain growth spurt did not influence the expression of this cotransporter protein.ConclusionI.V. general anaesthetics do not seem to influence developmental expression of KCC2 during the brain growth spurt. © 2013 © The Author [2013].
    Citation
    Lacoh, C.-M., Bodogan, T., Kaila, K., Fiumelli, H., & Vutskits, L. (2013). General anaesthetics do not impair developmental expression of the KCC2 potassium-chloride cotransporter in neonatal rats during the brain growth spurt. British Journal of Anaesthesia, 110, i10–i18. doi:10.1093/bja/aet063
    Sponsors
    This work was founded by the Swiss National Science Foundation (grant 31003A-130625 to L. V.) and by the Academy of Finland and the Letten Foundation (to K.K.).
    Publisher
    Elsevier BV
    Journal
    British Journal of Anaesthesia
    DOI
    10.1093/bja/aet063
    PubMed ID
    23533251
    ae974a485f413a2113503eed53cd6c53
    10.1093/bja/aet063
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division

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