General anaesthetics do not impair developmental expression of the KCC2 potassium-chloride cotransporter in neonatal rats during the brain growth spurt
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2013-03-27Online Publication Date
2013-03-27Print Publication Date
2013-06Permanent link to this record
http://hdl.handle.net/10754/562689
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BackgroundThe developmental transition from depolarizing to hyperpolarizing γ-aminobutyric acid-mediated neurotransmission is primarily mediated by an increase in the amount of the potassium-chloride cotransporter KCC2 during early postnatal life. However, it is not known whether early neuronal activity plays a modulatory role in the expression of total KCC2 mRNA and protein in the immature brain. As general anaesthetics are powerful modulators of neuronal activity, the purpose of this study was to explore how these drugs affect KCC2 expression during the brain growth spurt.MethodsWistar rat pups were exposed to either a single dose or 6 h of midazolam, propofol, or ketamine anaesthesia at postnatal days 0, 5, 10, or 15. KCC2 expression was assessed using immunoblotting, immunohistochemistry, or quantitative polymerase chain reaction analysis up to 3 days post-exposure in the medial prefrontal cortex.ResultsThere was a progressive and steep increase in the expression of KCC2 between birth and 2 weeks of age. Exposure to midazolam, propofol, or ketamine up to 6 h at any investigated stages of the brain growth spurt did not influence the expression of this cotransporter protein.ConclusionI.V. general anaesthetics do not seem to influence developmental expression of KCC2 during the brain growth spurt. © 2013 © The Author [2013].Citation
Lacoh, C.-M., Bodogan, T., Kaila, K., Fiumelli, H., & Vutskits, L. (2013). General anaesthetics do not impair developmental expression of the KCC2 potassium-chloride cotransporter in neonatal rats during the brain growth spurt. British Journal of Anaesthesia, 110, i10–i18. doi:10.1093/bja/aet063Sponsors
This work was founded by the Swiss National Science Foundation (grant 31003A-130625 to L. V.) and by the Academy of Finland and the Letten Foundation (to K.K.).Publisher
Elsevier BVJournal
British Journal of AnaesthesiaPubMed ID
23533251ae974a485f413a2113503eed53cd6c53
10.1093/bja/aet063
Scopus Count
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