UV and cold tolerance of a pigment-producing Antarctic Janthinobacterium sp. Ant5-2
Online Publication Date2013-03-20
Print Publication Date2013-05
Permanent link to this recordhttp://hdl.handle.net/10754/562687
MetadataShow full item record
AbstractIn this paper, we describe the UV and cold tolerance of a purple violet pigment (PVP)-producing Antarctic bacterium, Janthinobacterium sp. Ant5-2 (PVP+) and compared its physiological adaptations with a pigmentless mutant strain (PVP-). A spontaneous deletion of vioA that codes for tryptophan monooxygenase, the first gene involved in the biosynthesis of PVP was found in PVP- strain. The PVP- culture exhibited significantly reduced survival during exponential and stationary growth phase following exposure to UVB (320 nm) and UVC (254 nm) (dose range: 0-300 J/m2) when compared to wild-type (PVP+) cultures. In addition, upon biochemical inhibition of pigment synthesis by 2(5H)-furanone, wild-type PVP+ cultures exhibited approximately 50-fold growth reduction at a higher dose (300 J/m2) of UV. Increased resistance to UV was observed upon inducing starvation state in both PVP+ and PVP- cultures. There was 80 % (SD = ±8) reduction in extrapolymeric substance (EPS) production in the PVP- cultures along with a compromised survival to freeze-thaw cycles when compared to the PVP+ cultures. Perhaps synthesis of PVP and EPS are among the key adaptive features that define the survival of this bacterium in Antarctic extreme conditions, especially during austral summer months. © 2013 Springer Japan.
CitationMojib, N., Farhoomand, A., Andersen, D. T., & Bej, A. K. (2013). UV and cold tolerance of a pigment-producing Antarctic Janthinobacterium sp. Ant5-2. Extremophiles, 17(3), 367–378. doi:10.1007/s00792-013-0525-9
- Structure and function of a cold shock domain fold protein, CspD, in Janthinobacterium sp. Ant5-2 from East Antarctica.
- Authors: Mojib N, Andersen DT, Bej AK
- Issue date: 2011 Jun
- The antiproliferative function of violacein-like purple violet pigment (PVP) from an Antarctic Janthinobacterium sp. Ant5-2 in UV-induced 2237 fibrosarcoma.
- Authors: Mojib N, Nasti TH, Andersen DT, Attigada VR, Hoover RB, Yusuf N, Bej AK
- Issue date: 2011 Oct
- Antimycobacterial activity in vitro of pigments isolated from Antarctic bacteria.
- Authors: Mojib N, Philpott R, Huang JP, Niederweis M, Bej AK
- Issue date: 2010 Nov
- Psychrotrophic strain of Janthinobacterium lividum from a cold Alaskan soil produces prodigiosin.
- Authors: Schloss PD, Allen HK, Klimowicz AK, Mlot C, Gross JA, Savengsuksa S, McEllin J, Clardy J, Ruess RW, Handelsman J
- Issue date: 2010 Sep
- Draft Genome Sequence of Janthinobacterium sp. Ant5-2-1, Isolated from Proglacial Lake Podprudnoye in the Schirmacher Oasis of East Antarctica.
- Authors: Koo H, Strope BM, Kim EH, Shabani AM, Kumar R, Crowley MR, Andersen DT, Bej AK
- Issue date: 2016 Jan 21