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    Transcriptome and quantitative proteome analysis reveals molecular processes associated with larval metamorphosis in the polychaete pseudopolydora vexillosa

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    Type
    Article
    Authors
    Chandramouli, Kondethimmanahalli cc
    Sun, Jin
    Mok, FloraSy
    Liu, Lingli
    Qiu, Jian-Wen cc
    Ravasi, Timothy cc
    Qian, Pei-Yuan cc
    KAUST Department
    KAUST Global Collaborative Research Program
    Biological and Environmental Sciences and Engineering (BESE) Division
    Integrative Systems Biology Lab
    Bioscience Program
    Computational Bioscience Research Center (CBRC)
    Date
    2013-01-29
    Online Publication Date
    2013-01-29
    Print Publication Date
    2013-03
    Permanent link to this record
    http://hdl.handle.net/10754/562673
    
    Metadata
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    Abstract
    Larval growth of the polychaete worm Pseudopolydora vexillosa involves the formation of segment-specific structures. When larvae attain competency to settle, they discard swimming chaetae and secrete mucus. The larvae build tubes around themselves and metamorphose into benthic juveniles. Understanding the molecular processes, which regulate this complex and unique transition, remains a major challenge because of the limited molecular information available. To improve this situation, we conducted high-throughput RNA sequencing and quantitative proteome analysis of the larval stages of P. vexillosa. Based on gene ontology (GO) analysis, transcripts related to cellular and metabolic processes, binding, and catalytic activities were highly represented during larval-adult transition. Mitogen-activated protein kinase (MAPK), calcium-signaling, Wnt/β-catenin, and notch signaling metabolic pathways were enriched in transcriptome data. Quantitative proteomics identified 107 differentially expressed proteins in three distinct larval stages. Fourteen and 53 proteins exhibited specific differential expression during competency and metamorphosis, respectively. Dramatic up-regulation of proteins involved in signaling, metabolism, and cytoskeleton functions were found during the larval-juvenile transition. Several proteins involved in cell signaling, cytoskeleton and metabolism were up-regulated, whereas proteins related to transcription and oxidative phosphorylation were down-regulated during competency. The integration of high-throughput RNA sequencing and quantitative proteomics allowed a global scale analysis of larval transcripts/proteins associated molecular processes in the metamorphosis of polychaete worms. Further, transcriptomic and proteomic insights provide a new direction to understand the fundamental mechanisms that regulate larval metamorphosis in polychaetes. © 2013 American Chemical Society.
    Citation
    Chandramouli, K. H., Sun, J., Mok, F. S., Liu, L., Qiu, J.-W., Ravasi, T., & Qian, P.-Y. (2013). Transcriptome and Quantitative Proteome Analysis Reveals Molecular Processes Associated with Larval Metamorphosis in the Polychaete Pseudopolydora vexillosa. Journal of Proteome Research, 12(3), 1344–1358. doi:10.1021/pr3010088
    Sponsors
    The authors thank Dr. Salim Bougouffa for technical help in MG-RAST data analysis, Dr. Taewoo Ryu for reviewing the transcriptome data, Ms. Cherry Kwan for proofreading the manuscript, and Dr. Wang Yong for figures preparation. This study was supported by a grant from China Ocean Mineral Resources Research and Development (DY125-15-T-02), a grant from Sanya Institute of Deep-Sea Science and Engineering (SIDSSE-201206), and award SA-C0040/UK-00016 from the King Abdullah University of Science and Technology to PY Qian.
    Publisher
    American Chemical Society (ACS)
    Journal
    Journal of Proteome Research
    DOI
    10.1021/pr3010088
    PubMed ID
    23294167
    ae974a485f413a2113503eed53cd6c53
    10.1021/pr3010088
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Integrative Systems Biology Lab; Computational Bioscience Research Center (CBRC)

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