Transcriptome and quantitative proteome analysis reveals molecular processes associated with larval metamorphosis in the polychaete pseudopolydora vexillosa
KAUST DepartmentKAUST Global Collaborative Research Program
Biological and Environmental Sciences and Engineering (BESE) Division
Integrative Systems Biology Lab
Computational Bioscience Research Center (CBRC)
Online Publication Date2013-01-29
Print Publication Date2013-03
Permanent link to this recordhttp://hdl.handle.net/10754/562673
MetadataShow full item record
AbstractLarval growth of the polychaete worm Pseudopolydora vexillosa involves the formation of segment-specific structures. When larvae attain competency to settle, they discard swimming chaetae and secrete mucus. The larvae build tubes around themselves and metamorphose into benthic juveniles. Understanding the molecular processes, which regulate this complex and unique transition, remains a major challenge because of the limited molecular information available. To improve this situation, we conducted high-throughput RNA sequencing and quantitative proteome analysis of the larval stages of P. vexillosa. Based on gene ontology (GO) analysis, transcripts related to cellular and metabolic processes, binding, and catalytic activities were highly represented during larval-adult transition. Mitogen-activated protein kinase (MAPK), calcium-signaling, Wnt/β-catenin, and notch signaling metabolic pathways were enriched in transcriptome data. Quantitative proteomics identified 107 differentially expressed proteins in three distinct larval stages. Fourteen and 53 proteins exhibited specific differential expression during competency and metamorphosis, respectively. Dramatic up-regulation of proteins involved in signaling, metabolism, and cytoskeleton functions were found during the larval-juvenile transition. Several proteins involved in cell signaling, cytoskeleton and metabolism were up-regulated, whereas proteins related to transcription and oxidative phosphorylation were down-regulated during competency. The integration of high-throughput RNA sequencing and quantitative proteomics allowed a global scale analysis of larval transcripts/proteins associated molecular processes in the metamorphosis of polychaete worms. Further, transcriptomic and proteomic insights provide a new direction to understand the fundamental mechanisms that regulate larval metamorphosis in polychaetes. © 2013 American Chemical Society.
CitationChandramouli, K. H., Sun, J., Mok, F. S., Liu, L., Qiu, J.-W., Ravasi, T., & Qian, P.-Y. (2013). Transcriptome and Quantitative Proteome Analysis Reveals Molecular Processes Associated with Larval Metamorphosis in the Polychaete Pseudopolydora vexillosa. Journal of Proteome Research, 12(3), 1344–1358. doi:10.1021/pr3010088
SponsorsThe authors thank Dr. Salim Bougouffa for technical help in MG-RAST data analysis, Dr. Taewoo Ryu for reviewing the transcriptome data, Ms. Cherry Kwan for proofreading the manuscript, and Dr. Wang Yong for figures preparation. This study was supported by a grant from China Ocean Mineral Resources Research and Development (DY125-15-T-02), a grant from Sanya Institute of Deep-Sea Science and Engineering (SIDSSE-201206), and award SA-C0040/UK-00016 from the King Abdullah University of Science and Technology to PY Qian.
PublisherAmerican Chemical Society (ACS)
JournalJournal of Proteome Research
- Comparative glycoproteome analysis: dynamics of protein glycosylation during metamorphic transition from Pelagic to Benthic life stages in three invertebrates.
- Authors: Chandramouli KH, Zhang Y, Wong YH, Qian PY
- Issue date: 2012 Feb 3
- Phosphoproteome analysis during larval development and metamorphosis in the spionid polychaete Pseudopolydora vexillosa.
- Authors: Chandramouli KH, Mok FS, Wang H, Qian PY
- Issue date: 2011 May 25
- 2D gel-based multiplexed proteomic analysis during larval development and metamorphosis of the biofouling polychaete tubeworm Hydroides elegans.
- Authors: Zhang Y, Sun J, Xiao K, Arellano SM, Thiyagarajan V, Qian PY
- Issue date: 2010 Sep 3
- Involvement of a novel p38 mitogen-activated protein kinase in larval metamorphosis of the polychaete Hydroides elegans (Haswell).
- Authors: Wang H, Qian PY
- Issue date: 2010 Jul 15
- Proteomic profiling during the pre-competent to competent transition of the biofouling polychaete Hydroides elegans.
- Authors: Zhang Y, Sun J, Zhang H, Chandramouli KH, Xu Y, He LS, Ravasi T, Qian PY
- Issue date: 2014 Sep