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    A direct proofreader-clamp interaction stabilizes the Pol III replicase in the polymerization mode

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    Type
    Article
    Authors
    Jergic, Slobodan
    Horan, Nicholas P.
    Elshenawy, Mohamed cc
    Mason, Claire E.
    Urathamakul, Thitima
    Ozawa, Kiyoshi
    Robinson, Andrew J.
    Goudsmits, Joris M H
    Wang, Yao
    Pan, Xuefeng
    Beck, Jennifer L.
    Van Oijen, Antoine M.
    Huber, Thomas L.
    Hamdan, Samir cc
    Dixon, Nicholas E. cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Date
    2013-02-22
    Permanent link to this record
    http://hdl.handle.net/10754/562660
    
    Metadata
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    Abstract
    Processive DNA synthesis by the αÉ"θ core of the Escherichia coli Pol III replicase requires it to be bound to the β 2 clamp via a site in the α polymerase subunit. How the É" proofreading exonuclease subunit influences DNA synthesis by α was not previously understood. In this work, bulk assays of DNA replication were used to uncover a non-proofreading activity of É". Combination of mutagenesis with biophysical studies and single-molecule leading-strand replication assays traced this activity to a novel β-binding site in É" that, in conjunction with the site in α, maintains a closed state of the αÉ"θ-β 2 replicase in the polymerization mode of DNA synthesis. The É"-β interaction, selected during evolution to be weak and thus suited for transient disruption to enable access of alternate polymerases and other clamp binding proteins, therefore makes an important contribution to the network of protein-protein interactions that finely tune stability of the replicase on the DNA template in its various conformational states. © 2013 European Molecular Biology Organization.
    Citation
    Jergic, S., Horan, N. P., Elshenawy, M. M., Mason, C. E., Urathamakul, T., Ozawa, K., … Dixon, N. E. (2013). A direct proofreader–clamp interaction stabilizes the Pol III replicase in the polymerization mode. The EMBO Journal, 32(9), 1322–1333. doi:10.1038/emboj.2012.347
    Sponsors
    We thank Michelle Blayney and Linda Jessop for preliminary ESI-MS data. This work was supported by grants from the Australian Research Council, including Fellowships to KO, TH, and NED, and by a KAUST Faculty Initiated Collaborative grant to SMH and NED.
    Publisher
    Wiley
    Journal
    EMBO Journal
    DOI
    10.1038/emboj.2012.347
    PubMed ID
    23435564
    PubMed Central ID
    PMC3642676
    Additional Links
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3642676
    ae974a485f413a2113503eed53cd6c53
    10.1038/emboj.2012.347
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program

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